LOCUS       sequence-100663-         936 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector for cloning and expressing your gene 
            under the CAG promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Matsuda T, Cepko CL
  TITLE     Electroporation and RNA interference in the rodent retina in vivo 
            and in vitro.
  JOURNAL   Proc Natl Acad Sci U S A. 2004 Jan 6. 101(1):16-22.
  PUBMED    14603031
REFERENCE   2  (bases 1 to 936)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2004 Jan 6. 101(1):16-22."
COMMENT     Sequence Label: pCAGEN
FEATURES             Location/Qualifiers
     source          1..936
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(110..129)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    175..230
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(229..248)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(591..607)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(591..607)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(604..626)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    615..631
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(639..645,646..663,664..669))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    684..705
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ggcaaagaat tcctcgagga tatcgcggcc gcactcctca ggtgcaggct gcctatcaga
       61 aggtggtggc tggtgtggcc aatgccctgg ctcacaaata ccactgagat ctttttccct
      121 ctgccaaaaa ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa
      181 ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga
      241 catatgggag ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa
      301 catatgccca tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata
      361 tgaaacagcc ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga
      421 ttttttttat attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac
      481 atgttttact agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc
      541 ttctcttatg gagatccctc gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg
      601 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
      661 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
      721 ctgcccgctt tccagtcggg aaacctgtcg tgccagcgga tccgcatctc aattagtcag
      781 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
      841 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
      901 cctctgagct attccagaag tagtgaggag gctttt
//
