LOCUS       sequence-100664-         682 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector for cloning and expressing your gene 
            under the CAG promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Matsuda T, Cepko CL
  TITLE     Electroporation and RNA interference in the rodent retina in vivo 
            and in vitro.
  JOURNAL   Proc Natl Acad Sci U S A. 2004 Jan 6. 101(1):16-22.
  PUBMED    14603031
REFERENCE   2  (bases 1 to 682)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2004 Jan 6. 101(1):16-22."
COMMENT     Sequence Label: pCAGEN
FEATURES             Location/Qualifiers
     source          1..682
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(113..132)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(194..216)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 ttgtgagcca gggcattggc cacaccagcc accaccttct gataggcagc ctgcacctga
       61 ggagtgcggc cgcgatatcc tcgaggaatt ctttgccaaa atgatgagac agcacaataa
      121 ccagcacgtt gcccaggagc tgtaggaaaa agaagaaggc atgaacatgg ttagcagagg
      181 ctctagagcc gccggtcaca cgccagaagc cgaaccccgc cctgccccgt cccccccgaa
      241 ggcagccgtc cccccgcgga cagccccgag gctggagagg gagaagggga cggcggcgcg
      301 gcgacgcacg aaggccctcc ccgcccattt ccttcctgcc ggcgccgcac cgcttcgccc
      361 cgcgcccgct agagggggtg cggcggcgcc tcccagattt cggctccgca cagatttggg
      421 acaaaggaag tccctgcgcc ctctcgcacg attaccataa aaggcaatgg ctgcggctcg
      481 ccgcgcctcg acagccgccg gcgctccggg ggccgccgcg cccctccccc gagccctccc
      541 cggcccgagg cggccccgcc ccgcccggca cccccacctg ccgccacccc ccgcccggca
      601 cggcgagccc cgcgccacgc cccgtacgga gccccgcacc cgaagccggg ccgtgctcag
      661 caactcgggg aggggggtgc ag
//
