LOCUS       sequence-100682-         894 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SB-transposon with inducible SfiI cloning site for GOI (contains 
            firefly luciferase) and constitutive expression of BFP, rtTA and 
            neomycin resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Kowarz E, Loescher D, Marschalek R
  TITLE     Optimized Sleeping Beauty transposons rapidly generate stable 
            transgenic cell lines.
  JOURNAL   Biotechnol J. 2015 Feb 4. doi: 10.1002/biot.201400821.
  PUBMED    25650551
REFERENCE   2  (bases 1 to 894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2015 Feb 4. doi: 10.1002/biot.201400821."
COMMENT     Sequence Label: pSBtet-BN
FEATURES             Location/Qualifiers
     source          1..894
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             225..281
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 tgaagctcgt gggcgggagc catctgatcg caaacatcaa gaccacatat agatccaaga
       61 aacccgctaa gaacctcaag atgcctggcg tctactatgt ggactacaga ctggaaagaa
      121 tcaaggaggc caacaacgag acctacgtcg agcagcacga ggtggcagtg gccagatact
      181 gcgacctccc tagcaaactg gggcacaagc tgaacggcag tggagctact aacttcagcc
      241 tgctgaagca ggctggtgac gtcgaggaga atcctggccc catgtctaga ctggacaaga
      301 gcaaagtcat aaacggcgct ctggaattac tcaatggagt cggtatcgaa ggcctgacga
      361 caaggaaact cgctcaaaag ctgggagttg agcagcctac cctgtactgg cacgtgaaga
      421 acaagcgggc cctgctcgat gccctgccaa tcgagatgct ggacaggcat catacccact
      481 tctgccccct ggaaggcgag tcatggcaag actttctgcg gaacaacgcc aagtcattcc
      541 gctgtgctct cctctcacat cgcgacgggg ctaaagtgca tctcggcacc cgcccaacag
      601 agaaacagta cgaaaccctg gaaaatcagc tcgcgttcct gtgtcagcaa ggcttctccc
      661 tggagaacgc actgtacgct ctgtccgccg tgggccactt tacactgggc tgcgtattgg
      721 aggaacagga gcatcaagta gcaaaagagg aaagagagac acctaccacc gattctatgc
      781 ccccacttct gagacaagca attgagctgt tcgaccggca gggagccgaa cctgccttcc
      841 ttttcggcct ggaactaatc atatgtggcc tggagaaaca gctaaagtgc gaaa
//
