LOCUS       sequence-100683-         925 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SB-transposon with inducible SfiI cloning site for GOI (contains 
            firefly luciferase) and constitutive expression of BFP, rtTA and 
            neomycin resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Kowarz E, Loescher D, Marschalek R
  TITLE     Optimized Sleeping Beauty transposons rapidly generate stable 
            transgenic cell lines.
  JOURNAL   Biotechnol J. 2015 Feb 4. doi: 10.1002/biot.201400821.
  PUBMED    25650551
REFERENCE   2  (bases 1 to 925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2015 Feb 4. doi: 10.1002/biot.201400821."
COMMENT     Sequence Label: pSBtet-BN
FEATURES             Location/Qualifiers
     source          1..925
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             35..91
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(92..709,710..715,716..751,752..754,755..790,791..793,
                     794..829,830..835)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             842..898
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 tagcaaactg gggcacaagc tgaacggcag tggagctact aacttcagcc tgctgaagca
       61 ggctggtgac gtcgaggaga atcctggccc catgtctaga ctggacaaga gcaaagtcat
      121 aaacggcgct ctggaattac tcaatggagt cggtatcgaa ggcctgacga caaggaaact
      181 cgctcaaaag ctgggagttg agcagcctac cctgtactgg cacgtgaaga acaagcgggc
      241 cctgctcgat gccctgccaa tcgagatgct ggacaggcat catacccact tctgccccct
      301 ggaaggcgag tcatggcaag actttctgcg gaacaacgcc aagtcattcc gctgtgctct
      361 cctctcacat cgcgacgggg ctaaagtgca tctcggcacc cgcccaacag agaaacagta
      421 cgaaaccctg gaaaatcagc tcgcgttcct gtgtcagcaa ggcttctccc tggagaacgc
      481 actgtacgct ctgtccgccg tgggccactt tacactgggc tgcgtattgg aggaacagga
      541 gcatcaagta gcaaaagagg aaagagagac acctaccacc gattctatgc ccccacttct
      601 gagacaagca attgagctgt tcgaccggca gggagccgaa cctgccttcc ttttcggcct
      661 ggaactaatc atatgtggcc tggagaaaca gctaaagtgc gaaagcggcg ggccggccga
      721 cgcccttgac gattttgact tagacatgct cccagccgat gcccttgacg actttgacct
      781 tgatatgctg cctgctgacg ctcttgacga ttttgacctt gacatgctcc ccgggtccgg
      841 cgctactaac ttcagcctgc tgaagcaggc tggtgacgtc gaggagaatc ctggtcccat
      901 gattgaacaa gatggattgc acgca
//
