LOCUS       sequence-100684-         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SB-transposon with inducible SfiI cloning site for GOI (contains 
            firefly luciferase) and constitutive expression of RFP, rtTA and 
            puromycin resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Kowarz E, Loescher D, Marschalek R
  TITLE     Optimized Sleeping Beauty transposons rapidly generate stable 
            transgenic cell lines.
  JOURNAL   Biotechnol J. 2015 Feb 4. doi: 10.1002/biot.201400821.
  PUBMED    25650551
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2015 Feb 4. doi: 10.1002/biot.201400821."
COMMENT     Sequence Label: pSBtet-RP
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     136..159
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             252..308
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 gcgacggcgt gctgaagggc gagatccacc aggccctgaa gctgaaggac ggcggccact
       61 acctggtgga gttcaagacc atctacatgg ccaagaagcc cgtgcaactg cccggctact
      121 actacgtgga caccaagctg gacatcacct cccacaacga ggactacacc atcgtggaac
      181 agtacgagcg ctccgagggc cgccaccacc tgttcctgta cggcatggac gagctgtaca
      241 agggcagtgg agctactaac ttcagcctgc tgaagcaggc tggtgacgtc gaggagaatc
      301 ctggccccat gtctagactg gacaagagca aagtcataaa cggcgctctg gaattactca
      361 atggagtcgg tatcgaaggc ctgacgacaa ggaaactcgc tcaaaagctg ggagttgagc
      421 agcctaccct gtactggcac gtgaagaaca agcgggccct gctcgatgcc ctgccaatcg
      481 agatgctgga caggcatcat acccacttct gccccctgga aggcgagtca tggcaagact
      541 ttctgcggaa caacgccaag tcattccgct gtgctctcct ctcacatcgc gacggggcta
      601 aagtgcatct cggcacccgc ccaacagaga aacagtacga aaccctggaa aatcagctcg
      661 cgttcctgtg tcagcaaggc ttctccctgg agaacgcact gtacgctctg tccgccgtgg
      721 gccactttac actgggctgc gtattggagg aacaggagca tcaagtagca aaagaggaaa
      781 gagagacacc taccaccgat tctatgcccc cacttctgag acaagcaatt gagctgttcg
      841 accggcaggg agccgaacct gccttccttt tcggcctgga actaatc
//
