LOCUS       sequence-100686-         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SB-transposon with inducible SfiI cloning site for GOI (contains 
            firefly luciferase) and constitutive expression of BFP, rtTA and 
            puromycin resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Kowarz E, Loescher D, Marschalek R
  TITLE     Optimized Sleeping Beauty transposons rapidly generate stable 
            transgenic cell lines.
  JOURNAL   Biotechnol J. 2015 Feb 4. doi: 10.1002/biot.201400821.
  PUBMED    25650551
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2015 Feb 4. doi: 10.1002/biot.201400821."
COMMENT     Sequence Label: pSBtet-BP
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             231..287
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 tggccctgaa gctcgtgggc gggagccatc tgatcgcaaa catcaagacc acatatagat
       61 ccaagaaacc cgctaagaac ctcaagatgc ctggcgtcta ctatgtggac tacagactgg
      121 aaagaatcaa ggaggccaac aacgagacct acgtcgagca gcacgaggtg gcagtggcca
      181 gatactgcga cctccctagc aaactggggc acaagctgaa cggcagtgga gctactaact
      241 tcagcctgct gaagcaggct ggtgacgtcg aggagaatcc tggccccatg tctagactgg
      301 acaagagcaa agtcataaac ggcgctctgg aattactcaa tggagtcggt atcgaaggcc
      361 tgacgacaag gaaactcgct caaaagctgg gagttgagca gcctaccctg tactggcacg
      421 tgaagaacaa gcgggccctg ctcgatgccc tgccaatcga gatgctggac aggcatcata
      481 cccacttctg ccccctggaa ggcgagtcat ggcaagactt tctgcggaac aacgccaagt
      541 cattccgctg tgctctcctc tcacatcgcg acggggctaa agtgcatctc ggcacccgcc
      601 caacagagaa acagtacgaa accctggaaa atcagctcgc gttcctgtgt cagcaaggct
      661 tctccctgga gaacgcactg tacgctctgt ccgccgtggg ccactttaca ctgggctgcg
      721 tattggagga acaggagcat caagtagcaa aagaggaaag agagacacct accaccgatt
      781 ctatgccccc acttctgaga caagcaattg agctgttcga ccggcaggga gccgaacctg
      841 ccttcctttt cggcctggaa ctaatc
//
