LOCUS       sequence-100687-         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SB-transposon with inducible SfiI cloning site for GOI (contains 
            firefly luciferase) and constitutive expression of BFP, rtTA and 
            puromycin resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Kowarz E, Loescher D, Marschalek R
  TITLE     Optimized Sleeping Beauty transposons rapidly generate stable 
            transgenic cell lines.
  JOURNAL   Biotechnol J. 2015 Feb 4. doi: 10.1002/biot.201400821.
  PUBMED    25650551
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2015 Feb 4. doi: 10.1002/biot.201400821."
COMMENT     Sequence Label: pSBtet-BP
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             96..152
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(153..770,771..776,777..812,813..815,816..851,852..854,
                     855..890,891..896)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
ORIGIN
        1 ccaacaacga gacctacgtc gagcagcacg aggtggcagt ggccagatac tgcgacctcc
       61 ctagcaaact ggggcacaag ctgaacggca gtggagctac taacttcagc ctgctgaagc
      121 aggctggtga cgtcgaggag aatcctggcc ccatgtctag actggacaag agcaaagtca
      181 taaacggcgc tctggaatta ctcaatggag tcggtatcga aggcctgacg acaaggaaac
      241 tcgctcaaaa gctgggagtt gagcagccta ccctgtactg gcacgtgaag aacaagcggg
      301 ccctgctcga tgccctgcca atcgagatgc tggacaggca tcatacccac ttctgccccc
      361 tggaaggcga gtcatggcaa gactttctgc ggaacaacgc caagtcattc cgctgtgctc
      421 tcctctcaca tcgcgacggg gctaaagtgc atctcggcac ccgcccaaca gagaaacagt
      481 acgaaaccct ggaaaatcag ctcgcgttcc tgtgtcagca aggcttctcc ctggagaacg
      541 cactgtacgc tctgtccgcc gtgggccact ttacactggg ctgcgtattg gaggaacagg
      601 agcatcaagt agcaaaagag gaaagagaga cacctaccac cgattctatg cccccacttc
      661 tgagacaagc aattgagctg ttcgaccggc agggagccga acctgccttc cttttcggcc
      721 tggaactaat catatgtggc ctggagaaac agctaaagtg cgaaagcggc gggccggccg
      781 acgcccttga cgattttgac ttagacatgc tcccagccga tgcccttgac gactttgacc
      841 ttgatatgct gcctgctgac gctcttgacg attttgacct tgacatgctc cccgggtccg
      901 gcgctactaa cttcagcctg ctgaagcagg
//
