LOCUS       sequence-100688-         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SB-transposon with inducible SfiI cloning site for GOI (contains 
            firefly luciferase) and constitutive expression of BFP, rtTA and 
            blasticidin resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Kowarz E, Loescher D, Marschalek R
  TITLE     Optimized Sleeping Beauty transposons rapidly generate stable 
            transgenic cell lines.
  JOURNAL   Biotechnol J. 2015 Feb 4. doi: 10.1002/biot.201400821.
  PUBMED    25650551
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2015 Feb 4. doi: 10.1002/biot.201400821."
COMMENT     Sequence Label: pSBtet-BB
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             238..294
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 aacgacatgg ccctgaagct cgtgggcggg agccatctga tcgcaaacat caagaccaca
       61 tatagatcca agaaacccgc taagaacctc aagatgcctg gcgtctacta tgtggactac
      121 agactggaaa gaatcaagga ggccaacaac gagacctacg tcgagcagca cgaggtggca
      181 gtggccagat actgcgacct ccctagcaaa ctggggcaca agctgaacgg cagtggagct
      241 actaacttca gcctgctgaa gcaggctggt gacgtcgagg agaatcctgg ccccatgtct
      301 agactggaca agagcaaagt cataaacggc gctctggaat tactcaatgg agtcggtatc
      361 gaaggcctga cgacaaggaa actcgctcaa aagctgggag ttgagcagcc taccctgtac
      421 tggcacgtga agaacaagcg ggccctgctc gatgccctgc caatcgagat gctggacagg
      481 catcataccc acttctgccc cctggaaggc gagtcatggc aagactttct gcggaacaac
      541 gccaagtcat tccgctgtgc tctcctctca catcgcgacg gggctaaagt gcatctcggc
      601 acccgcccaa cagagaaaca gtacgaaacc ctggaaaatc agctcgcgtt cctgtgtcag
      661 caaggcttct ccctggagaa cgcactgtac gctctgtccg ccgtgggcca ctttacactg
      721 ggctgcgtat tggaggaaca ggagcatcaa gtagcaaaag aggaaagaga gacacctacc
      781 accgattcta tgcccccact tctgagacaa gcaattgagc tgttcgaccg gcagggagcc
      841 gaacctgcct tccttttcgg cctggaacta atc
//
