LOCUS       sequence-100691-         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SB-transposon with inducible SfiI cloning site for GOI (contains 
            firefly luciferase) and constitutive expression of GFP, rtTA and 
            neomycin resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Kowarz E, Loescher D, Marschalek R
  TITLE     Optimized Sleeping Beauty transposons rapidly generate stable 
            transgenic cell lines.
  JOURNAL   Biotechnol J. 2015 Feb 4. doi: 10.1002/biot.201400821.
  PUBMED    25650551
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2015 Feb 4. doi: 10.1002/biot.201400821."
COMMENT     Sequence Label: pSBtet-GN
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(57..674,675..680,681..716,717..719,720..755,756..758,
                     759..794,795..800)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             807..863
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 ctactaactt cagcctgctg aagcaggctg gtgacgtcga ggagaatcct ggccccatgt
       61 ctagactgga caagagcaaa gtcataaacg gcgctctgga attactcaat ggagtcggta
      121 tcgaaggcct gacgacaagg aaactcgctc aaaagctggg agttgagcag cctaccctgt
      181 actggcacgt gaagaacaag cgggccctgc tcgatgccct gccaatcgag atgctggaca
      241 ggcatcatac ccacttctgc cccctggaag gcgagtcatg gcaagacttt ctgcggaaca
      301 acgccaagtc attccgctgt gctctcctct cacatcgcga cggggctaaa gtgcatctcg
      361 gcacccgccc aacagagaaa cagtacgaaa ccctggaaaa tcagctcgcg ttcctgtgtc
      421 agcaaggctt ctccctggag aacgcactgt acgctctgtc cgccgtgggc cactttacac
      481 tgggctgcgt attggaggaa caggagcatc aagtagcaaa agaggaaaga gagacaccta
      541 ccaccgattc tatgccccca cttctgagac aagcaattga gctgttcgac cggcagggag
      601 ccgaacctgc cttccttttc ggcctggaac taatcatatg tggcctggag aaacagctaa
      661 agtgcgaaag cggcgggccg gccgacgccc ttgacgattt tgacttagac atgctcccag
      721 ccgatgccct tgacgacttt gaccttgata tgctgcctgc tgacgctctt gacgattttg
      781 accttgacat gctccccggg tccggcgcta ctaacttcag cctgctgaag caggctggtg
      841 acgtcgagga gaatcctggt cccatgattg aacaagatgg attgcacgca
//
