LOCUS       sequence-100693-         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SB-transposon with inducible SfiI cloning site for GOI (contains 
            firefly luciferase) and constitutive expression of GFP, rtTA and 
            blasticidin resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Kowarz E, Loescher D, Marschalek R
  TITLE     Optimized Sleeping Beauty transposons rapidly generate stable 
            transgenic cell lines.
  JOURNAL   Biotechnol J. 2015 Feb 4. doi: 10.1002/biot.201400821.
  PUBMED    25650551
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2015 Feb 4. doi: 10.1002/biot.201400821."
COMMENT     Sequence Label: pSBtet-GB
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             31..87
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(88..705,706..711,712..747,748..750,751..786,787..789,
                     790..825,826..831)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             838..894
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 ggcatggacg agctgtacaa gggcagtgga gctactaact tcagcctgct gaagcaggct
       61 ggtgacgtcg aggagaatcc tggccccatg tctagactgg acaagagcaa agtcataaac
      121 ggcgctctgg aattactcaa tggagtcggt atcgaaggcc tgacgacaag gaaactcgct
      181 caaaagctgg gagttgagca gcctaccctg tactggcacg tgaagaacaa gcgggccctg
      241 ctcgatgccc tgccaatcga gatgctggac aggcatcata cccacttctg ccccctggaa
      301 ggcgagtcat ggcaagactt tctgcggaac aacgccaagt cattccgctg tgctctcctc
      361 tcacatcgcg acggggctaa agtgcatctc ggcacccgcc caacagagaa acagtacgaa
      421 accctggaaa atcagctcgc gttcctgtgt cagcaaggct tctccctgga gaacgcactg
      481 tacgctctgt ccgccgtggg ccactttaca ctgggctgcg tattggagga acaggagcat
      541 caagtagcaa aagaggaaag agagacacct accaccgatt ctatgccccc acttctgaga
      601 caagcaattg agctgttcga ccggcaggga gccgaacctg ccttcctttt cggcctggaa
      661 ctaatcatat gtggcctgga gaaacagcta aagtgcgaaa gcggcgggcc ggccgacgcc
      721 cttgacgatt ttgacttaga catgctccca gccgatgccc ttgacgactt tgaccttgat
      781 atgctgcctg ctgacgctct tgacgatttt gaccttgaca tgctccccgg gtccggcgct
      841 actaacttca gcctgctgaa gcaggctggt gacgtcgagg agaatcctgg tcccatgg
//
