LOCUS       sequence-100696-         925 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SB-transposon with inducible SfiI cloning site for GOI (contains 
            firefly luciferase) and constitutive expression of RFP, rtTA and 
            hygromycin resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Kowarz E, Loescher D, Marschalek R
  TITLE     Optimized Sleeping Beauty transposons rapidly generate stable 
            transgenic cell lines.
  JOURNAL   Biotechnol J. 2015 Feb 4. doi: 10.1002/biot.201400821.
  PUBMED    25650551
REFERENCE   2  (bases 1 to 925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2015 Feb 4. doi: 10.1002/biot.201400821."
COMMENT     Sequence Label: pSBtet-RH
FEATURES             Location/Qualifiers
     source          1..925
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             79..135
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(136..753,754..759,760..795,796..798,799..834,835..837,
                     838..873,874..879)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
ORIGIN
        1 gtggaacagt acgagcgctc cgagggccgc caccacctgt tcctgtacgg catggacgag
       61 ctgtacaagg gcagtggagc tactaacttc agcctgctga agcaggctgg tgacgtcgag
      121 gagaatcctg gccccatgtc tagactggac aagagcaaag tcataaacgg cgctctggaa
      181 ttactcaatg gagtcggtat cgaaggcctg acgacaagga aactcgctca aaagctggga
      241 gttgagcagc ctaccctgta ctggcacgtg aagaacaagc gggccctgct cgatgccctg
      301 ccaatcgaga tgctggacag gcatcatacc cacttctgcc ccctggaagg cgagtcatgg
      361 caagactttc tgcggaacaa cgccaagtca ttccgctgtg ctctcctctc acatcgcgac
      421 ggggctaaag tgcatctcgg cacccgccca acagagaaac agtacgaaac cctggaaaat
      481 cagctcgcgt tcctgtgtca gcaaggcttc tccctggaga acgcactgta cgctctgtcc
      541 gccgtgggcc actttacact gggctgcgta ttggaggaac aggagcatca agtagcaaaa
      601 gaggaaagag agacacctac caccgattct atgcccccac ttctgagaca agcaattgag
      661 ctgttcgacc ggcagggagc cgaacctgcc ttccttttcg gcctggaact aatcatatgt
      721 ggcctggaga aacagctaaa gtgcgaaagc ggcgggccgg ccgacgccct tgacgatttt
      781 gacttagaca tgctcccagc cgatgccctt gacgactttg accttgatat gctgcctgct
      841 gacgctcttg acgattttga ccttgacatg ctccccgggt ccggcgctac taacttcagc
      901 ctgctgaagc aggctggtga cgtcg
//
