LOCUS       sequence-100699-         893 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SB-transposon with inducible SfiI cloning site for GOI (contains 
            firefly luciferase) and constitutive expression of rtTA and 
            puromycin resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Kowarz E, Loescher D, Marschalek R
  TITLE     Optimized Sleeping Beauty transposons rapidly generate stable 
            transgenic cell lines.
  JOURNAL   Biotechnol J. 2015 Feb 4. doi: 10.1002/biot.201400821.
  PUBMED    25650551
REFERENCE   2  (bases 1 to 893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2015 Feb 4. doi: 10.1002/biot.201400821."
COMMENT     Sequence Label: pSBtet-Pur
FEATURES             Location/Qualifiers
     source          1..893
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(126..743,744..749,750..785,786..788,789..824,825..827,
                     828..863,864..869)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
ORIGIN
        1 ggttcctgcg ttgggattcc gtgtacaatc catagacatc tgacctcggc acttagcatc
       61 atcacagcaa actaactgta gcctttctct ctttccctgt agaaacctct gcacctgagg
      121 ccaccatgtc tagactggac aagagcaaag tcataaacgg cgctctggaa ttactcaatg
      181 gagtcggtat cgaaggcctg acgacaagga aactcgctca aaagctggga gttgagcagc
      241 ctaccctgta ctggcacgtg aagaacaagc gggccctgct cgatgccctg ccaatcgaga
      301 tgctggacag gcatcatacc cacttctgcc ccctggaagg cgagtcatgg caagactttc
      361 tgcggaacaa cgccaagtca ttccgctgtg ctctcctctc acatcgcgac ggggctaaag
      421 tgcatctcgg cacccgccca acagagaaac agtacgaaac cctggaaaat cagctcgcgt
      481 tcctgtgtca gcaaggcttc tccctggaga acgcactgta cgctctgtcc gccgtgggcc
      541 actttacact gggctgcgta ttggaggaac aggagcatca agtagcaaaa gaggaaagag
      601 agacacctac caccgattct atgcccccac ttctgagaca agcaattgag ctgttcgacc
      661 ggcagggagc cgaacctgcc ttccttttcg gcctggaact aatcatatgt ggcctggaga
      721 aacagctaaa gtgcgaaagc ggcgggccgg ccgacgccct tgacgatttt gacttagaca
      781 tgctcccagc cgatgccctt gacgactttg accttgatat gctgcctgct gacgctcttg
      841 acgattttga ccttgacatg ctccccgggt ccggcgctac taacttcagc ctg
//
