LOCUS       sequence-100700-         935 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SB-transposon with inducible SfiI cloning site for GOI (contains 
            firefly luciferase) and constitutive expression of rtTA and neomycin
            resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Kowarz E, Loescher D, Marschalek R
  TITLE     Optimized Sleeping Beauty transposons rapidly generate stable 
            transgenic cell lines.
  JOURNAL   Biotechnol J. 2015 Feb 4. doi: 10.1002/biot.201400821.
  PUBMED    25650551
REFERENCE   2  (bases 1 to 935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2015 Feb 4. doi: 10.1002/biot.201400821."
COMMENT     Sequence Label: pSBtet-Neo
FEATURES             Location/Qualifiers
     source          1..935
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(102..719,720..725,726..761,762..764,765..800,801..803,
                     804..839,840..845)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             852..908
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 acaatccata gacatctgac ctcggcactt agcatcatca cagcaaacta actgtagcct
       61 ttctctcttt ccctgtagaa acctctgcac ctgaggccac catgtctaga ctggacaaga
      121 gcaaagtcat aaacggcgct ctggaattac tcaatggagt cggtatcgaa ggcctgacga
      181 caaggaaact cgctcaaaag ctgggagttg agcagcctac cctgtactgg cacgtgaaga
      241 acaagcgggc cctgctcgat gccctgccaa tcgagatgct ggacaggcat catacccact
      301 tctgccccct ggaaggcgag tcatggcaag actttctgcg gaacaacgcc aagtcattcc
      361 gctgtgctct cctctcacat cgcgacgggg ctaaagtgca tctcggcacc cgcccaacag
      421 agaaacagta cgaaaccctg gaaaatcagc tcgcgttcct gtgtcagcaa ggcttctccc
      481 tggagaacgc actgtacgct ctgtccgccg tgggccactt tacactgggc tgcgtattgg
      541 aggaacagga gcatcaagta gcaaaagagg aaagagagac acctaccacc gattctatgc
      601 ccccacttct gagacaagca attgagctgt tcgaccggca gggagccgaa cctgccttcc
      661 ttttcggcct ggaactaatc atatgtggcc tggagaaaca gctaaagtgc gaaagcggcg
      721 ggccggccga cgcccttgac gattttgact tagacatgct cccagccgat gcccttgacg
      781 actttgacct tgatatgctg cctgctgacg ctcttgacga ttttgacctt gacatgctcc
      841 ccgggtccgg cgctactaac ttcagcctgc tgaagcaggc tggtgacgtc gaggagaatc
      901 ctggtcccat gattgaacaa gatggattgc acgca
//
