LOCUS       sequence-100703-         921 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SB-transposon with inducible SfiI cloning site for GOI (contains 
            firefly luciferase) and constitutive expression of rtTA and 
            hygromycin resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Kowarz E, Loescher D, Marschalek R
  TITLE     Optimized Sleeping Beauty transposons rapidly generate stable 
            transgenic cell lines.
  JOURNAL   Biotechnol J. 2015 Feb 4. doi: 10.1002/biot.201400821.
  PUBMED    25650551
REFERENCE   2  (bases 1 to 921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2015 Feb 4. doi: 10.1002/biot.201400821."
COMMENT     Sequence Label: pSBtet-Hyg
FEATURES             Location/Qualifiers
     source          1..921
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(29..646,647..652,653..688,689..691,692..727,728..730,
                     731..766,767..772)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             779..835
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 tgtagaaacc tctgcacctg aggccaccat gtctagactg gacaagagca aagtcataaa
       61 cggcgctctg gaattactca atggagtcgg tatcgaaggc ctgacgacaa ggaaactcgc
      121 tcaaaagctg ggagttgagc agcctaccct gtactggcac gtgaagaaca agcgggccct
      181 gctcgatgcc ctgccaatcg agatgctgga caggcatcat acccacttct gccccctgga
      241 aggcgagtca tggcaagact ttctgcggaa caacgccaag tcattccgct gtgctctcct
      301 ctcacatcgc gacggggcta aagtgcatct cggcacccgc ccaacagaga aacagtacga
      361 aaccctggaa aatcagctcg cgttcctgtg tcagcaaggc ttctccctgg agaacgcact
      421 gtacgctctg tccgccgtgg gccactttac actgggctgc gtattggagg aacaggagca
      481 tcaagtagca aaagaggaaa gagagacacc taccaccgat tctatgcccc cacttctgag
      541 acaagcaatt gagctgttcg accggcaggg agccgaacct gccttccttt tcggcctgga
      601 actaatcata tgtggcctgg agaaacagct aaagtgcgaa agcggcgggc cggccgacgc
      661 ccttgacgat tttgacttag acatgctccc agccgatgcc cttgacgact ttgaccttga
      721 tatgctgcct gctgacgctc ttgacgattt tgaccttgac atgctccccg ggtccggcgc
      781 tactaacttc agcctgctga agcaggctgg tgacgtcgag gagaatcctg gtcccatgaa
      841 aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt
      901 ctccgacctg atgcagctct c
//
