LOCUS       sequence-10072-e       13610 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13610)
  AUTHORS   Novoa I, Zeng H, Harding HP, Ron D
  TITLE     Feedback inhibition of the unfolded protein response by 
            GADD34-mediated dephosphorylation of eIF2alpha.
  JOURNAL   J Cell Biol. 2001 May 28. 153(5):1011-22.
  PUBMED    11381086
REFERENCE   2  (bases 1 to 13610)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2001 May 28. 153(5):1011-22."
COMMENT     Sequence Label: CHOP::GFP
FEATURES             Location/Qualifiers
     source          1..13610
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             6455..6466
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     regulatory      9763..9772
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(9769..9771,9772..9774,9775..10488)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(9769..9771,9772..9774,9775..10485)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9814..9835)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(10075..10094)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     10422..10443
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    10528..10649
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10897..10914)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11068..11656)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11148..11167)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11827..12618,12619..12687))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12450..12469
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12688..12792)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      12819..13274
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12906..12925)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13116..13137
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    13464..13585
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 cccgggaggt accgagctct tacgcgtatc gatggcgcca gctgcaggcg gccgccnggt
       61 cgacnagctt ccatccgagt gggaagggcc aggggtgggg agtcccagta gggacagcac
      121 agaggggaga ataggctggg atggctctgg gagaggaaag ggctgaggga caggagcagg
      181 ggcccgagga cgagtccgtc taggggtctg agggcctccc ccttctagca accgcaagac
      241 agttgggggc cggcgaggcc gccgctgaga gggacggggt gatgcatggg cagctgaggg
      301 tgcctgggca cggggccttc ggccctgtaa agtgctggga gggggcagct gggccttggc
      361 tgctgcagag agcagactgc tagcaaggaa tgggggtgcc ccaggcccct ccactgcggg
      421 ggcccctccc aaggatccca ggaccaaggg caggtgcata gtggctgaag acactggtgg
      481 ctgagtggca ggaccagggg ggccaggggg accagggggg ttattgcttg ggggcagggg
      541 aggagatgtt aaggcatcgc tacagtggaa gagaggagag tctgagggtg gtgaagagga
      601 ggcgtgaggg gctggaggag agcccaggtc agagggcacc aggttggatc ggagggaagc
      661 cccccagttg taggaggggg cattgaggct gatagcaggt gggggaggtg gggctggaga
      721 gggagcattg cctcttggga ggaaacaagg gctgctgctg cctgcagagg gaactggatc
      781 aggaagccgg ggtggtggga agagccctgc agggcctgtc agagggggga aggcctgggg
      841 aacggaaggt gggtcttggg gaatggagcc aggacccccg ttaagtggag ttgtaggagg
      901 agctcgacaa ggagggcgga cagagggggg ccctggggac acagtgtgga acatctgggg
      961 gcttgctccc tctcctgcaa taaatgagaa gtcaagggtc agcttgtggg tccatccctg
     1021 cctcgaatgc tgtcttctcc agtcccagtg cccacaagtc acaaccatgt cctttctcac
     1081 caagctgccc ccactgactc caccctgggc ctgccttctc agatcacctc ccccacatcc
     1141 accttccagg ccccaggttt cccccatttt tctctaaagt ggcaggaaaa ctcaccagga
     1201 gaagagtgtg agcaggtggt ctccatgctg cggtacagag ttgccatggc aacagctttc
     1261 cgcctgtggt tgcacagctt ggtcatgtcc tcctctgatg caggccccac cccagcccca
     1321 cccggggtca ccggggccag agggtcaaag ttgaaaacct gtaaggtagg aagcatggac
     1381 agggacagtg agggggtggg atctcaggga ggcacggtgg tttgtaattg gcttatgcat
     1441 tgagtctctg gcacccccac agctctgacc ttgggtacat taagtggaca ctccagaccg
     1501 cacttgcagg tcccatcgct gaggaggtag ctacgggttt gctccaagga agacagctct
     1561 gtgccacttg ggctgggaaa gaacagggtc acaagtatgg ggttaatctc agcctatctg
     1621 gagacacttc ctaaaggaca gttaaatgct ttcctgggaa gggcaaggcg gaaaggacat
     1681 gggaagacac aaagagtgaa tggatccaga gacgcagaga tacagagagc aacaaggtgc
     1741 caggaaccac agctctgaga ggaggggtga ggggacgaaa actctgaaaa gccatacctg
     1801 atatagtaca cagcaccttc tcgcacacag cgttgccacc cgatggggat ggggacaggc
     1861 gtggcggcag ggcccccagc tctgtctgct gcactgctcg cattgtctcc attcattgtg
     1921 tgtgatgagc tcccctgtgc ctgacaacac agacatccat gtgggcacga ggacgcttac
     1981 accgggctgg gtacctgagg gagagttctg tcataccacc atggctacct gaacatctct
     2041 gcaaacattg tgggatccag tctaaagccg gggccaccag cttagcccac acctgcaata
     2101 caaggaaaga gaagggcctc tcagtctacc aactcaagaa acaccacagc ccagagcact
     2161 caaggaaagg accataaata acatgacgta gcgcctgtaa ggacctcagg atctgctggc
     2221 catgtccaca acggtcccct ccaagcccag tccctagaga ccagaggagt tcagcacctc
     2281 aactcccaag gaccacaaaa ccattcccta atttcttgac atatgaaggc ctagttccct
     2341 ttgtctaggc acagaagact tcagctagga gtgtgggcaa attcccataa tgccaggcca
     2401 ctaggaagtt tcccagtgtc tggccactgc tgtgccctcc cccaggaaga tcatataaaa
     2461 agtacagatt gccccacagg tcaccaagca attgcccaac tgcctggcag ggtagtgcca
     2521 gctgaaatcc ccccatgccc actccatcca acaatctcgg atctaattca tttggacctc
     2581 caaagaggct ggagccagag ctctgtcttc ctccaggatc ctgagaactc ctggtcctct
     2641 caagccgacg aaaaggggat tcataatccc ccactggctt ccaattaaca acaagaagca
     2701 ttccctgaca tgtagggacg gcccttagac ctgcaagtac ccctcaccaa cctcaccatc
     2761 gctcccgccg cacactgacg gcttccaaac tttccctctc ataacaaggc gccctgtcac
     2821 ccagactgct tccttcagct cggggaggag gggaaggcag gcgcacgaag taggaaggaa
     2881 cttggggaga gggcgggtgg agggtgggcg aagcactgaa gggagcgagg tcaggaaggc
     2941 agaagtcagg cactttggga ggagaaatgg cgggggcagg tgagggtgcg gggctgtgga
     3001 tggggccagg gaaaggggcc gaaaggacgc ggagggggca gagggtaggg acagaagggg
     3061 aggagagaga agggaggggg aggggcgggg gggccgggcc ccgcactcac cgcggcccat
     3121 ggttcggctg gccccgccct gcgcagtcgc ggcccagagg gtgagtggga gggggtggga
     3181 ggtgggtcgg tggagcccga gcctccggta cggccccggc cggtcttagc aggaagcgcc
     3241 gccgccgccg ccgccgcgga tcaccgagcg gcctcccgcg catgcgccat aggggccagg
     3301 ggcagccctg gggattccgt tcccagaagg caccgcgcag gctgctaccg ccgccgccgt
     3361 cgctgccgcc gccaccgccg cagccgccgc cgccgccgcc gccgctgccc ggacgggaga
     3421 ggggcggggc cgggcccccg cccagcggac agcagcgagc caacaggagg ggacgcagtg
     3481 cgcatgcggg agcgcgccta cccctccccc acaacccccc attaatcccc aagagaacaa
     3541 acagcggcgg gggccccccc ccactcgccc tccgcggaag gatctgggcc tcttcccagg
     3601 ccactggtgg ccaatcccag tcctccctcg ggaggggcgc cttccgaagc cacaatctgt
     3661 tgggggagtc aggaatgtca ggtccgggag gggccagccc taaaagatga aagtcgcgac
     3721 ttgccctgcc ccgccccaaa ggcttcccgg gtagtgtgca gagacttgac ccgcctctcc
     3781 aggtcaacat gtcacaacac gacctcagcc tgtcaagtca catgacctct gcctgtcact
     3841 gacaacctgg tctgtcttta ggccaggaga gaccatctgg tccaacccac cccccacctc
     3901 tactaacaga aggggaaatg aaggtctgga gcgaaggagc agtctggcca aggacctgtc
     3961 accgtcgcac catccaatag caaccggtct tggttcagtt tctacttaaa ctctggcttg
     4021 cccctcttga gtctacgaca gactatgtaa taggccacca catggcctct gttttcatgg
     4081 cacaaaaaaa aaaaaatacg ttttgcaaga tgtttttact tttactcaag ttttctccac
     4141 agagctgctt tttaccttta tcgttgttta atttacaaat gagtaaggta gaatccttgg
     4201 acaaattaaa ctagaatctg tgtttcctct gatgacccag tccagggcaa tcatgcgttc
     4261 aagtgcatgc acgtcccagg ctctactgta ggtcgtgaac tcagacagct agaggcagga
     4321 ggccagctcc tcagcaggga gtcacaggac tcgctatctc ggagaaggaa gagggtggat
     4381 actgggctta gtgaagggcg taagagcatc acacaaagca ggggtgggat gaggaggagg
     4441 cagcaatcta cactctgtaa agaacttcaa tcctagcacg taatggacga ttcagaaaca
     4501 agcgttaaag gtgcctctct gagagaagcg ggtggactat caaggaactc agtctttggg
     4561 acgcattaga aggctaaaag aggaggagag ctcttaaaag attcgaggag atgtcaatcc
     4621 aggtgaacaa attggaaact ttaaagaggt tccagagccc cggtgcccat tagctgggag
     4681 gggccaggga aaatgggagc ctttcatgga ggagtaagtt aaaggtgatg ggaggtgggc
     4741 agacaagttc aggaaggaca gccgttgggg ccgttggaca ctgggagctg gcgctccgcc
     4801 ctctttcctc tctcccccat agcccccctc ccgcacctcc caccaccatc gacgtcccct
     4861 gcgcgtgcgc gcgcggacac cggttgccaa acattgcatc atccccgccc ccccatcccc
     4921 tccctcgccg cgctctcctc cgcgcgcgcg catgactcac tcacctcctc cgcgaagccg
     4981 cgtgacccaa agccacttcc gggtccgaga caacgtagac tctcaagcca gagggcgcga
     5041 tgggggtggg gccgtgagac tcctgagtgg cggatgtaag ggatgaggcg gagccagtgc
     5101 cagcgtgccg ctttctgatt ggtaggctcc tggaccctgc ccccccccca ggaggggccg
     5161 gacagcataa aggatactcg ctctccgctc ttgaggtcag ttatcttgag cctaacacgt
     5221 cgattatatc atgttgaaga tgagcgggtg gcagcgacag agccagaata acagccggaa
     5281 cctgaggaga gaggcgagta ctggctccgt ctaaccctta ccttcccatc ctggccagca
     5341 tagctatttt ataaagttgg agacaactgt tctggaggat gtagccccac cccttccact
     5401 gtaagcttgt agactgggaa gggtgaaggc cgggaatccc tgattcagga atggtgctta
     5461 caggccctct cttgcattgg gcgattgcat tcccgttccc ttgtctcgag ttggtcccca
     5521 ctctgcagag ggactccggt tgccttcggg aacttgtctc tgtagtgcga actgcgggcc
     5581 agcttaggcc tgggcagttc gcaggaaagg cggggcagca ggacacaccc ccaaagtcgg
     5641 ggactctggc tgtctcagcc ttttcttcac cagcctcatt ccccccaact ctttcaagct
     5701 gaagcgcctc agtcaagggg ccaacatggc cacgttagta gctgactctc aagttcaaga
     5761 gaacgcgaag actgacagat ctttgcgagt gggggtgctg gtgggaggag catagatggg
     5821 tttttccctt ttccccagag gtttctaggt ttccatacaa gagagcagcc tggatctctt
     5881 taactatggg aaattatttg ggatctctcc agggtcttct gcttctccca gggctgattg
     5941 atctctgctt ctttcctcag tcccgtctcc taactaaact gtgtaaccat tgcgtcaggc
     6001 cagcccactg tttgtctctg caccctcagg cctggggctc ctctgctgat gaaagccagg
     6061 tcccacacac tggaagacca agggagtttt ccctagagga caaccctgca gagaaacact
     6121 cagggcaata ttgcatctgc agtccccaga gacagccagc tccgctctgc ctttaggctc
     6181 agtgggctta attctctata agggaacttg ggggaggggc tttttcccca ttcattccta
     6241 acaaatgcct aataagcacc cactatgtac caagtatctg gggacccagg gtatattagg
     6301 agcaaaagat aaaactgttc tcaataaaac ttacatttca gtataaagca aaagggaaaa
     6361 aaaaaacaaa agcagggcag aatagttcag ggcggtgact agaggttgtc atcagaagcc
     6421 aagcctgtga gccaagcccc ggtgttagag ctttatagaa gggagaggct gagcaacaga
     6481 gttaagataa gcatgccatg ccggtatctt taggaacagg actagagtaa gcaggagagg
     6541 gtgatgaaag gaaactcaag cagccggtct catagagcct tgcaagcacc ctcatgatgc
     6601 taatctttaa caatcatgaa acaggaagcc attgggggat ctgagcagag gaatgatcat
     6661 agcacatgca tttaattcca gcactcagag aggcagatct ctgagctcca ggccagccag
     6721 ggctacatta tgagaccctg actcaaaata aatacaaaaa caaagggtta ggtatgtagc
     6781 tcaatagtac actgcttgcc tggtatgtag aggaccctag gtcaacccac agccgtgaaa
     6841 ataaagtaaa agacaagaat agaaacagga ggtctcagct gaacatagtg gcacagcctt
     6901 taatcccagc acttgaaagg caggcagatc taaattcaaa gccagagagt ctacaaagca
     6961 agttccagga cagcaaaagc tacatagtga gaccctgtct aaaagagaaa gaaaggaaag
     7021 gaaaggaaga aagaaaggag agatttcaaa gaggcttcca aaataatcca gggaaagaaa
     7081 tgtatggtaa atttatatct ggagccaggc agtggtggtg cacgccttta atcccagcat
     7141 ttgggagaca gagacaggcg gatttctgaa ttcgaggcca gccttgtcca cagtgagttc
     7201 caggacagcc aaggctacac agagaaaccc tgtcaagaaa aaccaaaaaa ataaataaat
     7261 gaaaaataaa aaattgcatc tggaaataat gtagtaatga gaaatactta ggtttggggt
     7321 acttttaaag ttgtcagtgc tttaggctga gctgtggtat ctgagaaagg ggcatcaagg
     7381 ataccaaagt ttttggcttt cagcacctag agttgccatt tagtaagaac tatacagcct
     7441 tggtaggaaa ggcctttcat gtaagctgaa cgcctgctca acactgccaa ggagctggag
     7501 atcccagagc aggtctgcac tgaggctgcc agtgggggga tggaatctga gcaagcactg
     7561 ctgagtttac tcttccttcc ctctagttct gtctcccttt ggcggacttg aggtctatct
     7621 gggatttaga gaaaacttag ttggccagga ctttgctatt atgtaatcaa gacggcaaat
     7681 gtcagcaact aaaaataaag ttaagcctgc ctctgtcctt ggagttggct tcagggacac
     7741 acacgctgca agacacacgc ctgtgtgtgt gtgtgtatta caagagaaga aatggcttca
     7801 gggacacacc cgctgcaaga cacacacacc tctgtatgtg tgtgtgtgta ttacaagaga
     7861 agaactggag cctggtctgc agagttgttg agttccagaa ttccaggatg cccagggatg
     7921 cacaaagaaa ccatctcaca acccatttta gaaaggatct agaaaagggc taggaggagt
     7981 tagaaaggat ctagagaggg gctaggagga gttagaaagg atctagagag gggctaggag
     8041 gagttagaaa ggatctagag aagggctagg aggaggctgg aggtaaagga ctgttttttc
     8101 cttttctcca cagtgttcca gaaggaagtg catcttcata caccaccaca cctgaaagca
     8161 ggtaatcttg gccaccctct cagtcagctg cgaatgctgc tcatgttgct ttgctggggg
     8221 agagttagac aaggccttca tctgaacaag cttttgctgt cagaccatgt ctttctctga
     8281 ccccataatg ttgtttgaga gctggctttc gaagtcagcc tcaacaatgg caattggttt
     8341 gtcttcccaa ctctcagtcc acaccaagat tagacccaaa ggaccttttg cccaagcttc
     8401 ccacacgaga gaaaaaaaga gtacaaatgg cctggtaatt gcccctggaa attaccagta
     8461 gtgttcccaa gagagttgaa tacttttact gtaatcctgt aagaatatat atgtatagcc
     8521 aagcccagtg actgttcctc tctcagaact tgagaggatc ctgaagttat ctgtagtaaa
     8581 tccttctcaa taaagggtat gttctatcag gtgtagtggt gcataccttt agtcccagca
     8641 cttgggaggc agaggaaggt aaatgtctga gtcaaagcca gcctattttg cagagtgagt
     8701 tccagggaag ccagaactat atatacagag aaaccctttc ttgaaaatac accccccacc
     8761 ctccaagttt tgtttccagg agctagagag atgactcagt ggttaagagc acgtgctctt
     8821 cttgtagagg atctagtcta actcccagta cccaaatggc agttcaaaac catctatgac
     8881 tctagttccc agggaatcca ttccctcttg tggcctccat gtctaccagg aacacacaca
     8941 cagcacacat tcaggcactc acttatatat gcatagataa taaaataaat atatctttgg
     9001 gaaaaaaaaa aagatattta aggttaggta gccagcctgg attaagcttg gtagtgacca
     9061 gcatataaat gaaaacaaaa caaaaagttg gaaagctgtg tgggggtggt gcatactttt
     9121 aatcccagcc ttcaggaggc tgaggcaggt tgatctctga ggccagtgtg agttctagga
     9181 caggcagggc tacacagaga gaccctgtct tgaacaaaca ccaaaaagaa tggcaatgag
     9241 agcccggaga aagcctatca gttccacacc catgctgcct gtgtgccgta cctgagtcag
     9301 gtttccagca gccacagaag gtggctcaca tggcctggac ctccagctcc aggagagcca
     9361 atgaatgctg ctggccccca gacactgaat tacatccgtt tcagggtcct ggccatggtg
     9421 tgcatgtgat catctggaca acttttgaga gttggatctg gcagggtcaa agtcaaggct
     9481 gctaggcttg agaggcagcc atctccccat cccgacacac catcattagt gtgtgtgcag
     9541 gtcagagaac aacttgtgcg agttgactct tcacctccac cctctgccaa tgtagccttc
     9601 aaggagtgac aacccatgcc cttacctatc gtgcaagacc agtaaatttt aaattctacg
     9661 tgttagaaaa gggacaaggt cagctcaccg actgtggtga atggaatgta tgtcctttcc
     9721 agaacctggt ccacctagca ttcaggtcga gatccaccgg tcgccaccat ggtgagcaag
     9781 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     9841 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     9901 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     9961 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
    10021 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
    10081 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
    10141 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
    10201 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
    10261 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
    10321 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
    10381 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
    10441 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaatc tagagtcggg
    10501 gcggccggcc gcttcgagca gacatgataa gatacattga tgagtttgga caaaccacaa
    10561 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
    10621 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc
    10681 aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta
    10741 aaatcgataa ggatccgtcg accgatgccc ttgagagcct tcaacccagt cagctccttc
    10801 cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa
    10861 ctcgtaggac aggtgccggc agcgctcttc cgcttcctcg ctcactgact cgctgcgctc
    10921 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
    10981 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
    11041 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
    11101 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
    11161 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
    11221 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta
    11281 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
    11341 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
    11401 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
    11461 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
    11521 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
    11581 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
    11641 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
    11701 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
    11761 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
    11821 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
    11881 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
    11941 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
    12001 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
    12061 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
    12121 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
    12181 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
    12241 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
    12301 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
    12361 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
    12421 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
    12481 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
    12541 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
    12601 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
    12661 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
    12721 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
    12781 aggggttccg cgcacatttc cccgaaaagt gccacctgac gcgccctgta gcggcgcatt
    12841 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
    12901 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
    12961 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
    13021 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
    13081 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
    13141 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
    13201 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
    13261 aacgtttaca atttcccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
    13321 gcgggcctct tcgctattac gccagcccaa gctaccatga taagtaagta atattaaggt
    13381 acgtggaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
    13441 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
    13501 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
    13561 tgtccaaact catcaatgta tcttatggta ctgtaactga gctaacataa
//
