LOCUS       sequence-100724-        8452 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of human SNRNP70 fused to monomeric streptavidin 
            (N-terminus).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8452)
  AUTHORS   Beck DB, Narendra V, Drury WJ 3rd, Casey R, Jansen PW, Yuan ZF, 
            Garcia BA, Vermeulen M, Bonasio R
  TITLE     In Vivo Proximity Labeling for the Detection of Protein-Protein and 
            Protein-RNA Interactions.
  JOURNAL   J Proteome Res. 2014 Dec 5;13(12):6135-43. doi: 10.1021/pr500196b. 
            Epub 2014 Oct 29.
  PUBMED    25311790
REFERENCE   2  (bases 1 to 8452)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/pr500196b";
            journalName: "J Proteome Res"; date: "2014-12-5- 5"; volume: "13"; 
            issue: "12"; pages: "6135-43"
COMMENT     Sequence Label: pINTO-NSA::hSNRNP70
FEATURES             Location/Qualifiers
     source          1..8452
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(52..71)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1223..1602
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1603..1806
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1757..1777
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    1808..1826
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1829..1847
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1857..1881
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      1969..1978
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(3839..3856)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3845..4069
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4115..4543
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4202..4221)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4412..4433
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4552..4572)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4557..4886
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4737..4872
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4799..4818
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4934..4981
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5000..5374
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5504..5625
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5541..5560)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5595..5614
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5674..5690)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5674..5690)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5687..5709)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5698..5714
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5722..5728,5729..5746,5747..5752))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5767..5788
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6537..6554)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6708..7293)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6788..6807)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7464..8255,8256..8324))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8087..8106
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8325..8429)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgacgtcga cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg
       61 ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga
      121 gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgttaatt
      181 aagctagcag tctgcaatga gttcgagttg gcgcgcctgt cattctaaat ctctctttca
      241 gcctaaagct ttttccccgt atccccccag gtgtctgcag gctcaaagag cagcgagaag
      301 cgttcagagg aaagcgatcc cgtgccacct tccccgtgcc cgggctgtcc ccgcacgctg
      361 ccggctcggg gatgcggggg gagcgccgga ccggagcgga gccccgggcg gctcgctgct
      421 gccccctagc gggggaggga cgtaattaca tccctggggg ctttgggagg gggctgtccc
      481 cgtgagctcc caggcgcgcc tgtcattcta aatctctctt tcagcctaaa gctttttccc
      541 cgtatccccc caggtgtctg caggctcaaa gagcagcgag aagcgttcag aggaaagcga
      601 tcccgtgcca ccttccccgt gcccgggctg tccccgcacg ctgccggctc ggggatgcgg
      661 ggggagcgcc ggaccggagc ggagccccgg gcggctcgct gctgccccct agcgggggag
      721 ggacgtaatt acatccctgg gggctttggg ggggggctgt ccccgtgagc tcccaggcgc
      781 gccaactcgg tactcgggga tgctagcgca ccatcaacga ggaggacatc ttggctaacc
      841 aacccctgtt gttacagtcc atccaggaca gacttggctc gctggtgggc caggacagcg
      901 ggtatgtggg gggtcttccc aagaacgtta aggaaaagct gctgagcttg aagactttac
      961 aaagcgagct attcgaagtt gaaaaggaat ttcaggtgga gatgtttgag ctagagaaca
     1021 agtttctgca gaagtacaag cccatttggg agcagcggtc caggatcatt tcaggacaag
     1081 agcaacccaa gccggaacag atcgcaaagg gccaagagat tgtggagtca ctcaatgaga
     1141 cggagttgca attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat
     1201 gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata gtaatcaatt
     1261 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
     1321 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     1381 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     1441 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
     1501 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
     1561 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
     1621 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
     1681 gacgtcaatg ggagtttgtt ttggaaccaa aatcaacggg actttccaaa atgtcgtaac
     1741 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
     1801 agagctctcc ctatcagtga tagagatctc cctatcagtg atagagatcg tcgacgagct
     1861 cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga
     1921 agacaccggg accgatccag cctccggact ctagcgttta aacttaaggc caccatgggc
     1981 gaccccagca aagattctaa agcccaggtg tctgccgccg aagccggaat tacaggcaca
     2041 tggtataatc agctgggatc tacatttatt gtgacagccg gcgccgatgg agccctgaca
     2101 ggaacatatg aatctgccgt gggaaatgcc gaatccagat acaccctgac aggaagatat
     2161 gattctgccc ctgccacaga tggatctgga acagccctgg gatggagagt ggcctggaag
     2221 aataattata gaaacgccca tagcgccaca acatggtctg gccagtatgt gggaggcgcc
     2281 gaagccagaa ttaacacaca gtggaccctg acatctggaa caacagaagc caatgcctgg
     2341 aaaagcactc tgagaggaca tgatacattt acaaaagtga aacctagcgc cgcctctatc
     2401 gatgccgcca agaaggccgg agtgaacaat ggcaatcctc tggatgccgt gcagcagacc
     2461 ggtagcggta ccgagctcgg atccatgacc cagttcctgc cgcccaacct tctggccctc
     2521 tttgcccccc gtgaccctat tccatacctg ccacccctgg agaaactgcc acatgaaaaa
     2581 caccacaatc aaccttattg tggcattgcg ccgtacattc gagagtttga ggacccccga
     2641 gatgcccctc ctccaactcg tgctgaaacc cgagaggagc gcatggagag gaaaagacgg
     2701 gaaaagattg agcggcgaca gcaagaagtg gagacagagc ttaaaatgtg ggaccctcac
     2761 aatgatccca atgctcaggg ggatgccttc aagactctct tcgtggcgag agtgaattat
     2821 gacacaacag aatccaagct ccggagagag tttgaggtgt acggacctat caaaagaata
     2881 cacatggtct acagtaagcg gtcaggaaag ccccgtggct atgccttcat cgagtacgaa
     2941 cacgagcgag acatgcactc cgcttacaaa cacgcagatg gcaagaagat tgatggcagg
     3001 agggtccttg tggacgtgga gaggggccga accgtgaagg gctggaggcc ccggcggcta
     3061 ggaggaggcc tcggtggtac cagaagagga ggggctgatg tgaacatccg gcattcaggc
     3121 cgcgatgaca cctcccgcta cgatgagagg cccggcccct ccccgcttcc gcacagggac
     3181 cgggaccggg accgtgagcg ggagcgcaga gagcggagcc gggagcgaga caaggagcga
     3241 gaacggcgac gctcccgctc ccgggaccgg cggaggcgct cacggagtcg cgacaaggag
     3301 gagcggaggc gctccaggga gcggagcaag gacaaggacc gggaccggaa gcggcgaagc
     3361 agccggagtc gggagcgggc ccggcgggag cgggagcgca aggaggagct gcgtggtggc
     3421 ggtggcgaca tggcggagcc ctccgaggcg ggtgacgcgc cccctgatga tgggcctcca
     3481 ggggagctcg ggcctgacgg ccctgacggt ccagaggaaa agggccggga tcgtgaccgg
     3541 gagcgacggc ggagccaccg gagcgagcgc gagcggcgcc gggaccggga tcgtgaccgt
     3601 gaccgtgacc gcgagcacaa acggggggag cggggcagtg agcggggcag ggatgaggcc
     3661 cgaggtgggg gcggtggcca ggacaacggg ctggagggtc tgggcaacga cagccgagac
     3721 atgtacatgg agtctgaggg cggcgacggc tacctggctc cggagaatgg gtatttgatg
     3781 gaggctgcgc cggagtgatg actcgagtct agagggcccg tttaaacccg ctgatcagcc
     3841 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     3901 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     3961 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     4021 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
     4081 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc
     4141 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     4201 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     4261 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     4321 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     4381 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     4441 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     4501 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt
     4561 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     4621 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     4681 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     4741 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     4801 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct
     4861 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat
     4921 ctgatcagca cgtgttgaca attaatcatc ggcatagtat atcggcatag tataatacga
     4981 caaggtgagg aactaaacca tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg
     5041 cgacgtcgcc ggagcggtcg agttctggac cgaccggctc gggttctccc gggacttcgt
     5101 ggaggacgac ttcgccggtg tggtccggga cgacgtgacc ctgttcatca gcgcggtcca
     5161 ggaccaggtg gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct
     5221 gtacgccgag tggtcggagg tcgtgtccac gaacttccgg gacgcctccg ggccggccat
     5281 gaccgagatc ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa
     5341 ctgcgtgcac ttcgtggccg aggagcagga ctgacacgtg ctacgagatt tcgattccac
     5401 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
     5461 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc
     5521 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     5581 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc
     5641 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     5701 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     5761 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     5821 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     5881 gcgtattggg cgctcttccg ctagtctgca atgagttcga gttggcgcgc ctgtcattct
     5941 aaatctctct ttcagcctaa agctttttcc ccgtatcccc ccaggtgtct gcaggctcaa
     6001 agagcagcga gaagcgttca gaggaaagcg atcccgtgcc accttccccg tgcccgggct
     6061 gtccccgcac gctgccggct cggggatgcg gggggagcgc cggaccggag cggagccccg
     6121 ggcggctcgc tgctgccccc tagcggggga gggacgtaat tacatccctg ggggctttgg
     6181 gagggggctg tccccgtgag ctcccaggcg cgcctgtcat tctaaatctc tctttcagcc
     6241 taaagctttt tccccgtatc cccccaggtg tctgcaggct caaagagcag cgagaagcgt
     6301 tcagaggaaa gcgatcccgt gccaccttcc ccgtgcccgg gctgtccccg cacgctgccg
     6361 gctcggggat gcggggggag cgccggaccg gagcggagcc ccgggcggct cgctgctgcc
     6421 ccctagcggg ggagggacgt aattacatcc ctgggggctt tggggggggg ctgtccccgt
     6481 gagctcccag gcgcgccaac tcggtactcg gggatttaat taagctcttc cgcttcctcg
     6541 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     6601 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     6661 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     6721 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     6781 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     6841 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     6901 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     6961 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     7021 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     7081 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     7141 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     7201 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtttttt tgtttgcaag
     7261 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     7321 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     7381 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     7441 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     7501 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     7561 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     7621 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     7681 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     7741 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     7801 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     7861 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     7921 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     7981 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     8041 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     8101 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     8161 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     8221 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     8281 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     8341 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     8401 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc ac
//
