LOCUS       sequence-100727-         946 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Deuschle K, Okumoto S, Fehr M, Looger LL, Kozhukh L, Frommer WB
  TITLE     Construction and optimization of a family of genetically encoded 
            metabolite sensors by semirational protein engineering.
  JOURNAL   Protein Sci. 2005 Sep . 14(9):2304-14.
  PUBMED    16131659
REFERENCE   2  (bases 1 to 946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci. 2005 Sep . 14(9):2304-14."
COMMENT     Sequence Label: pRSET FLII275Pglu-4.6m
FEATURES             Location/Qualifiers
     source          1..946
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(551..802)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(593..614)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 actgtaactg ttcagttttg atgcctttat cgttcaattc tttaatcacg taagtggtac
       61 gtgcttctgc atccggatgg cccggttcac ctttcagcag tacgaactga atctgaccgt
      121 ctttgttcag atcccaaccc tgattcgccg cccagtgttt agcaatcaaa tcgccttgaa
      181 taatgccgga ctctttggag tcagtgccaa cgtagtaggc tttgtcgtag ctatccagcg
      241 ccttacgaga cggttctttg ttgaagaaaa ccaccggcac gttttgccca cgcgctttct
      301 caatcaccgt acccgcagct gccgggtcaa ccaggttgat tgccagtgcc ttcacccctt
      361 tcgccagcaa tacgtcgatc tgatcgttct gcttggactg gtcattctga gaatcattca
      421 tcagcagctg aacatctggc gcggctttcg catcttgctc aatagccttg cgcactacag
      481 acatcgcgtt atcgtcgtac ttatagattg ttacaccaat gcgagtatca gcggcgcctc
      541 cggtaccacc cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact
      601 ccagcaggac catgtgatcg cgcttctcgt tggggtcttt gctcagggcg gactggtagc
      661 tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt
      721 agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg aagttcacct
      781 tgatgccgtt cttctgcttg tcggccatga tatagacgtt gtggctgttg tagttgtact
      841 ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc
      901 ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg ggtctt
//
