LOCUS       sequence-100728-         926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Deuschle K, Okumoto S, Fehr M, Looger LL, Kozhukh L, Frommer WB
  TITLE     Construction and optimization of a family of genetically encoded 
            metabolite sensors by semirational protein engineering.
  JOURNAL   Protein Sci. 2005 Sep . 14(9):2304-14.
  PUBMED    16131659
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci. 2005 Sep . 14(9):2304-14."
COMMENT     Sequence Label: pRSET FLII275Pglu-4.6m
FEATURES             Location/Qualifiers
     source          1..926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(222..224,225..227,228..740)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(267..288)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(528..547)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     875..896
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tcgaagtggt tatcgccaac aacgatgcga tggcaatggg cgcggttgaa gcgctgaaag
       61 cacacaacaa gtccagcatt ccggtgtttg gcgtcgatgc gctgccagaa gcgctggcgc
      121 tggtgaaatc cggtgcactg gcgggcaccg tactgaacga tgctaacaac caggcgaaag
      181 cgacctttga tctggcgaaa aacctggccg attcggctgg tatggtgagc aagggcgagg
      241 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
      301 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
      361 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
      421 ggggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
      481 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
      541 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
      601 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
      661 tcagccacaa cgtctatatc accgccgaca agcagaagaa cggcatcaag gccaacttca
      721 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
      781 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
      841 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
      901 ccgccgggat cactctcggc atggac
//
