LOCUS       sequence-100729-        1066 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression vector of rat Asip guide RNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Yoshimi K, Kaneko T, Voigt B, Mashimo T
  TITLE     Allele-specific genome editing and correction of disease-associated 
            phenotypes in rats using the CRISPR-Cas platform.
  JOURNAL   Nat Commun. 2014 Jun 26;5:4240. doi: 10.1038/ncomms5240.
  PUBMED    24967838
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-06-26"; volume: "5:4240. doi"; pages: " 
            10.1038/ncomms5240"
COMMENT     Sequence Label: pT7-gRNA:Asip
FEATURES             Location/Qualifiers
     source          1..1066
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     26..43
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     27..43
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_RNA        complement(225..300)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(321..339)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(468..484)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(468..484)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      532..563
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        564..666
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(740..759)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 tttctaccga agaaaggccc acccgtgtaa aacgacggcc agtttatcta gtcagcttga
       61 ttctagctga tcgtggaccg gaaggtgagc cagtgagttg attgcagtcc agttacgctg
      121 gagtctgagg ctcgtcctga atgatatgcg accgccggag ggttgcgttt gagacgggcg
      181 acagatccag tcgcgctgct ctcgtcgatc caagcttttt aaaagcaccg actcggtgcc
      241 actttttcaa gttgataacg gactagcctt attttaactt gctatttcta gctctaaaac
      301 gtgagttaga ctcctgtcat cctatagtga gtcgtattag ctagcggtgc gagcggatcg
      361 agcagtgtcg atcactactg gaccgcgagc tgtgctgcga cccgtgatct tacggcatta
      421 tacgtatgat cggtccacga tcagctagat tatctagtca gcttgatgtc atagctgttt
      481 cctgaggctc aatactgacc atttaaatca tacctgacct ccatagcaga aagtcaaaag
      541 cctccgaccg gaggcttttg acttgatcgg cacgtaagag gttccaactt tcaccataat
      601 gaaataagat cactaccggg cgtatttttt gagttatcga gattttcagg agctaaggaa
      661 gctaaaatga gccatattca acgggaaacg tcttgcttga agccgcgatt aaattccaac
      721 atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg
      781 acaatctatc gattgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa
      841 ggtagcgttg ccaatgatgt tacagatgag atggtcaggc taaactggct gacggaattt
      901 atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc
      961 actgcgatcc cagggaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa
     1021 aatattgttg atgcgctggc agtgttcctg cgccggttgc attcga
//
