LOCUS       sequence-100730-        1042 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression vector of rat Asip guide RNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Yoshimi K, Kaneko T, Voigt B, Mashimo T
  TITLE     Allele-specific genome editing and correction of disease-associated 
            phenotypes in rats using the CRISPR-Cas platform.
  JOURNAL   Nat Commun. 2014 Jun 26;5:4240. doi: 10.1038/ncomms5240.
  PUBMED    24967838
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-06-26"; volume: "5:4240. doi"; pages: " 
            10.1038/ncomms5240"
COMMENT     Sequence Label: pT7-gRNA:Asip
FEATURES             Location/Qualifiers
     source          1..1042
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(58..160)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(161..192)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     primer_bind     240..256
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     240..256
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        385..403
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_RNA        424..499
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(681..698)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(681..697)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 catgttggaa tttaatcgcg gcttcaagca agacgtttcc cgttgaatat ggctcatttt
       61 agcttcctta gctcctgaaa atctcgataa ctcaaaaaat acgcccggta gtgatcttat
      121 ttcattatgg tgaaagttgg aacctcttac gtgccgatca agtcaaaagc ctccggtcgg
      181 aggcttttga ctttctgcta tggaggtcag gtatgattta aatggtcagt attgagcctc
      241 aggaaacagc tatgacatca agctgactag ataatctagc tgatcgtgga ccgatcatac
      301 gtataatgcc gtaagatcac gggtcgcagc acagctcgcg gtccagtagt gatcgacact
      361 gctcgatccg ctcgcaccgc tagctaatac gactcactat aggatgacag gagtctaact
      421 cacgttttag agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa
      481 agtggcaccg agtcggtgct tttaaaaagc ttggatcgac gagagcagcg cgactggatc
      541 tgtcgcccgt ctcaaacgca accctccggc ggtcgcatat cattcaggac gagcctcaga
      601 ctccagcgta actggactgc aatcaactca ctggctcacc ttccggtcca cgatcagcta
      661 gaatcaagct gactagataa actggccgtc gttttacacg ggtgggcctt tcttcggtag
      721 aaaatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
      781 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
      841 ccgaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
      901 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
      961 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     1021 gatagttacc ggataaggcg ca
//
