LOCUS       sequence-100732-         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression vector for Cre-recombinase containing MET15 
            selection marker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  AUTHORS   MacDonald C, Piper RC
  TITLE     Puromycin and Methotrexate Resistance Cassettes and Optimized 
            cre-recombinase Expression Plasmids for use in Yeast.
  JOURNAL   Yeast. 2015 Feb 16. doi: 10.1002/yea.3069.
  PUBMED    25688547
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2015
            Feb 16. doi: 10.1002/yea.3069."
COMMENT     Sequence Label: pPL5628_TEF1*-Cre_MET15
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(4..251)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     217..235
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
ORIGIN
        1 gccgcaaatt aaagccttcg agcgtcccaa aaccttctca agcaaggttt tcagtataat
       61 gttacatgcg tacacgcgtc tgtacagaaa aaaaagaaaa atttgaaata taaataacgt
      121 tcttaatact aacataacta taaaaaaata aatagggacc tagacttcag gttgtctaac
      181 tccttccttt tcggttagag cggatgtggg gggagggcgt gaatgtaagc gtgacataac
      241 taattacatg actcgaggtc gacaattcca accttaccca agagttcgcc aaactcagac
      301 atcactttag caaaaccgcg ccgtgcttct tcctcggtgg cattcatcac gaaatgttca
      361 gcactacgca tacttttgac aggaaacgca acggatattg agtcaatatc aggcattcta
      421 tcgctcagct ttacagtgac aatgacggct ggcgactgaa tattagtgct tacagacagc
      481 actacatatt ttccgtcgat gttgaaatcc tttctcatat gtcaccataa atatcaaata
      541 attatagcaa tcatttacgc gttaatggct aatcgccatc ttccagcagg cgcaccattg
      601 cccctgtttc actatccagg ttacggatat agttcatgac aatatttaca ttggtccagc
      661 caccagcttg catgatctcc ggtattgaaa ctccagcgcg ggccatatct cgcgcggctc
      721 cgacacgggc actgtgtcca gaccaggcca ggtatctctg accagagtca tccttagcgc
      781 cgtaaatcaa tcgatgagtt gcttcaaaaa tcccttccag ggcgcgagtt gatagctggc
      841 tggtggcaga tggcgcggc
//
