LOCUS       sequence-100735-        1049 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression vector for Cre-recombinase containing ADE2 
            selection marker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   MacDonald C, Piper RC
  TITLE     Puromycin and Methotrexate Resistance Cassettes and Optimized 
            cre-recombinase Expression Plasmids for use in Yeast.
  JOURNAL   Yeast. 2015 Feb 16. doi: 10.1002/yea.3069.
  PUBMED    25688547
REFERENCE   2  (bases 1 to 1049)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2015
            Feb 16. doi: 10.1002/yea.3069."
COMMENT     Sequence Label: pPL5606_TEF1*-Cre_ADE2
FEATURES             Location/Qualifiers
     source          1..1049
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     199..221
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     213..230
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     214..230
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     240..259
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        240..258
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      complement(277..524)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     490..508
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
ORIGIN
        1 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
       61 ctacagggcg cgtccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
      121 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
      181 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gagcgcgcgt
      241 aatacgactc actatagggc gaattgggta ccggccgcaa attaaagcct tcgagcgtcc
      301 caaaaccttc tcaagcaagg ttttcagtat aatgttacat gcgtacacgc gtctgtacag
      361 aaaaaaaaga aaaatttgaa atataaataa cgttcttaat actaacataa ctataaaaaa
      421 ataaataggg acctagactt caggttgtct aactccttcc ttttcggtta gagcggatgt
      481 ggggggaggg cgtgaatgta agcgtgacat aactaattac atgactcgag gtcgacaatt
      541 ccaaccttac ccaagagttc gccaaactca gacatcactt tagcaaaacc gcgccgtgct
      601 tcttcctcgg tggcattcat cacgaaatgt tcagcactac gcatactttt gacaggaaac
      661 gcaacggata ttgagtcaat atcaggcatt ctatcgctca gctttacagt gacaatgacg
      721 gctggcgact gaatattagt gcttacagac agcactacat attttccgtc gatgttgaaa
      781 tcctttctca tatgtcacca taaatatcaa ataattatag caatcattta cgcgttaatg
      841 gctaatcgcc atcttccagc aggcgcacca ttgcccctgt ttcactatcc aggttacgga
      901 tatagttcat gacaatattt acattggtcc agccaccagc ttgcatgatc tccggtattg
      961 aaactccagc gcgggccata tctcgcgcgg ctccgacacg ggcactgtgt ccagaccagg
     1021 ccaggtatct ctgaccagag tcatcctta
//
