LOCUS       sequence-100741-         731 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  to express human AID recoded for yeast codon usage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Mayorov VI, Rogozin IB, Adkison LR, Frahm C, Kunkel TA, Pavlov YI
  TITLE     Expression of human AID in yeast induces mutations in context 
            similar to the context of somatic hypermutation at G-C pairs in 
            immunoglobulin genes.
  JOURNAL   BMC Immunol. 2005 Jun 10;6:10.
  PUBMED    15949042
REFERENCE   2  (bases 1 to 731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Immunol."; date: "2005-06-10"; volume: "6"; pages: "10"
COMMENT     Sequence Label: pESC-LEU-HsAIDSc
FEATURES             Location/Qualifiers
     source          1..731
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             10..603
                     /codon_start=1
                     /gene="AICDA"
                     /product="human activation-induced cytidine deaminase 
                     (Dickerson et al., 2003)"
                     /label=AID
                     /translation="MDSLLMNRRKFLYQFKNVRWAKGRRETYLCYVVKRRDSATSFSLD
                     FGYLRNKNGCHVELLFLRYISDWDLDPGRCYRVTWFTSWSPCYDCARHVADFLRGNPNL
                     SLRIFTARLYFCEDRKAEPEGLRRLHRAGVQIAIMTFKDYFYCWNTFVENHERTFKAWE
                     GLHENSVRLSRQLRRILLPLYEVDDLRDAFRTLGL"
     CDS             613..642
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 ccggatccga tggactcttt gttgatgaat agaagaaagt ttttgtacca atttaagaac
       61 gtcagatggg ctaagggtag aagagaaacc tacttgtgtt acgtcgttaa gagaagagac
      121 tctgctactt ctttctcctt ggacttcggt tacttgagaa acaaaaacgg ttgtcacgtc
      181 gaattgctat tcttgagata catctctgat tgggacttgg acccaggtag atgttacaga
      241 gttacttggt tcacttcttg gtccccttgt tacgactgtg ccagacacgt tgccgacttc
      301 ttgagaggta acccaaactt gtccttgaga atcttcaccg ctcgtttgta cttctgtgaa
      361 gacagaaagg ctgaaccaga aggtttgaga agattgcaca gagctggtgt tcaaattgcc
      421 atcatgacct tcaaggacta cttctactgc tggaacactt tcgttgaaaa ccatgaaaga
      481 accttcaagg cttgggaagg tttgcacgag aactccgtca gattgagccg tcaattgaga
      541 agaattttgt taccattata tgaagtcgat gatttgagag atgctttcag aaccttgggt
      601 ttagtcgaca tggaacagaa gttgatttcc gaagaagacc tcgagtaagc ttggtaccgc
      661 ggctagctaa gatccgctct aaccgaaaag gaaggagtta gacaacctga agtctaggtc
      721 cctatttatt t
//
