LOCUS       sequence-100742-         645 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lentiviral vector for GATA1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 645)
  AUTHORS   Elcheva I, Brok-Volchanskaya V, Kumar A, Liu P, Lee JH, Tong L, 
            Vodyanik M, Swanson S, Stewart R, Kyba M, Yakubov E, Cooke J, 
            Thomson JA, Slukvin I
  TITLE     Direct induction of haematoendothelial programs in human pluripotent
            stem cells by transcriptional regulators.
  JOURNAL   Nat Commun. 2014 Jul 14;5:4372. doi: 10.1038/ncomms5372.
  PUBMED    25019369
REFERENCE   2  (bases 1 to 645)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-07-14"; volume: "5:4372. doi"; pages: " 
            10.1038/ncomms5372"
COMMENT     Sequence Label: pSIN4-EF1a-GATA1-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..645
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      16..25
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             25..48
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gtgtcgtgag gattcgccac catggactac aaggacgacg atgacaagga gttccctggc
       61 ctggggtccc tggggacctc agagcccctc ccccagtttg tggatcctgc tctggtgtcc
      121 tccacaccag aatcaggggt tttcttcccc tctgggcctg agggcttgga tgcagcagct
      181 tcctccactg ccccgagcac agccaccgct gcagctgcgg cactggccta ctacagggac
      241 gctgaggcct acagacactc cccagtcttt caggtgtacc cattgctcaa ctgtatggag
      301 gggatcccag ggggctcacc atatgccggc tgggcctacg gcaagacggg gctctaccct
      361 gcctcaactg tgtgtcccac ccgcgaggac tctcctcccc aggccgtgga agatctggat
      421 ggaaaaggca gcaccagctt cctggagact ttgaagacag agcggctgag cccagacctc
      481 ctgaccctgg gacctgcact gccttcatca ctccctgtcc ccaatagtgc ttatgggggc
      541 cctgactttt ccagtacctt cttttctccc accgggagcc ccctcaattc agcagcctat
      601 tcctctccca agcttcgtgg aactctcccc ctgcctccct gtgag
//
