LOCUS       sequence-100743-         582 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lentiviral vector for GATA1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Elcheva I, Brok-Volchanskaya V, Kumar A, Liu P, Lee JH, Tong L, 
            Vodyanik M, Swanson S, Stewart R, Kyba M, Yakubov E, Cooke J, 
            Thomson JA, Slukvin I
  TITLE     Direct induction of haematoendothelial programs in human pluripotent
            stem cells by transcriptional regulators.
  JOURNAL   Nat Commun. 2014 Jul 14;5:4372. doi: 10.1038/ncomms5372.
  PUBMED    25019369
REFERENCE   2  (bases 1 to 582)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-07-14"; volume: "5:4372. doi"; pages: " 
            10.1038/ncomms5372"
COMMENT     Sequence Label: pSIN4-EF1a-GATA1-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..582
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    8..581
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(168..187)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     397..414
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 tggcagctta tcatcgtgtt tttcaaagga aaaccacgtc cccgtggttc ggggggccta
       61 gacgtttttt taacctcgac taaacacatg taaagcatgt gcaccgaggc cccagatcag
      121 atcccataca atggggtacc ttctgggcat ccttcagccc cttgttgaat acgcttgagg
      181 agagccattt gactctttcc acaactatcc aactcacaac gtggcactgg ggttgtgccg
      241 cctttgcagg tgtatcttat acacgtggct tttggccgca gaggcacctg tcgccaggtg
      301 gggggttccg ctgcctgcaa agggtcgcta cagacgttgt ttgtcttcaa gaagcttcca
      361 gaggaactgc ttccttcacg acattcaaca gaccttgcat tcctttggcg agaggggaaa
      421 gacccctagg aatgctcgtc aagaagacag ggccaggttt ccgggccctc acattgccaa
      481 aagacggcaa tatggtggaa aataacatat agacaaacgc acaccggcct tattccaagc
      541 ggcttcggcc agtaacgtta gggggggggg agggagaggg gg
//
