LOCUS       sequence-100744-         573 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lentiviral vector for ETV2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Elcheva I, Brok-Volchanskaya V, Kumar A, Liu P, Lee JH, Tong L, 
            Vodyanik M, Swanson S, Stewart R, Kyba M, Yakubov E, Cooke J, 
            Thomson JA, Slukvin I
  TITLE     Direct induction of haematoendothelial programs in human pluripotent
            stem cells by transcriptional regulators.
  JOURNAL   Nat Commun. 2014 Jul 14;5:4372. doi: 10.1038/ncomms5372.
  PUBMED    25019369
REFERENCE   2  (bases 1 to 573)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-07-14"; volume: "5:4372. doi"; pages: " 
            10.1038/ncomms5372"
COMMENT     Sequence Label: pSIN4-EF1a-ETV2-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..573
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    9..471
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(169..188)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     398..415
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 gtggcagctt atcatcgtgt ttttcaaagg aaaaccacgt ccccgtggtt cggggggcct
       61 agacgttttt ttaacctcga ctaaacacat gtaaagcatg tgcaccgagg ccccagatca
      121 gatcccatac aatggggtac cttctgggca tccttcagcc ccttgttgaa tacgcttgag
      181 gagagccatt tgactctttc cacaactatc caactcacaa cgtggcactg gggttgtgcc
      241 gcctttgcag gtgtatctta tacacgtggc ttttggccgc agaggcacct gtcgccaggt
      301 ggggggttcc gctgcctgca aagggtcgct acagacgttg tttgtcttca agaagcttcc
      361 agaggaactg cttccttcac gacattcaac agaccttgca ttcctttggc gagaggggaa
      421 agacccctag gaatgctcgt caagaagaca gggccaggtt tccgggccct cacattgcca
      481 aaagacggca atatggtgga aaataacata tagacaaacg cacaccggcc ttattccaag
      541 cggcttcggc cagtaacgtt aggggggggg gag
//
