LOCUS       sequence-100745-         582 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  to overexpress yeast polymerase eta in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Pavlov YI, Nguyen D, Kunkel TA
  TITLE     Mutator effects of overproducing DNA polymerase eta (Rad30) and its 
            catalytically inactive variant in yeast.
  JOURNAL   Mutat Res. 2001 Jul 1;478(1-2):129-39.
  PUBMED    11406177
REFERENCE   2  (bases 1 to 582)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mutat 
            Res."; date: "2001-07-1"; volume: "478(1-2)"; pages: "129-39"
COMMENT     Sequence Label: pESC-LEU2-RAD30cmyc
FEATURES             Location/Qualifiers
     source          1..582
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     10..29
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        10..28
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ccggatccgt aatacgactc actatagggc ccatgtcaaa atttacttgg aaggagttga
       61 ttcagcttgg ttcccccagt aaagcatacg agtcctcctt agcatgtatc gcccacatag
      121 atatgaatgc cttttttgca caggttgagc agatgcgatg tggcttaagc aaagaagatc
      181 cggtagtctg cgttcagtgg aattccatta ttgcagtctc ttatgcagca agaaagtatg
      241 gtatatcaag gatggatacg attcaggaag cactaaaaaa atgtagtaac ctcataccga
      301 tccatacagc cgtttttaaa aagggagaag atttttggca atatcatgat ggatgtggat
      361 cttgggtgca ggatcctgca aagcagataa gtgtggaaga tcataaagta tcactggaac
      421 catataggag ggaaagtcgc aaggcgctga agattttcaa aagcgcatgt gatctagtcg
      481 aaagggcgag tattgatgaa gtatttcttg atttgggccg gatttgtttc aatatgctaa
      541 tgtttgataa tgagtacgag cttacaggcg acttgaaact ga
//
