LOCUS       sequence-100756-         798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Genetically encoded fluorescent voltage indicator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Mishina Y, Mutoh H, Song C, Knopfel T
  TITLE     Exploration of genetically encoded voltage indicators based on a 
            chimeric voltage sensing domain.
  JOURNAL   Front Mol Neurosci. 2014 Sep 29;7:78. doi: 10.3389/fnmol.2014.00078.
            eCollection 2014.
  PUBMED    25324718
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Mol 
            Neurosci."; date: "2014-09-29"; volume: "7:78. doi"; pages: " 
            10.3389/fnmol.2014.00078. eCollection 2014"
COMMENT     Sequence Label: pCAG-Chimeric_Butterfly_YR_1.0
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(92..343)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(134..155)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     483..502
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     742..763
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gcattggcca caccagccac caccttctga taggcagcct gcacctgagg agtgcggccg
       61 ccttaagctt ggtaccgagc tcggatcctt acttgtacag ctcgtccatg ccgagagtga
      121 tcccggcggc ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg ttggggtctt
      181 tgctcagttt ggactggtag ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc
      241 cgatgggggt gttctgctgg tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt
      301 ggcggatctt gaagttcacc ttgatgccgt tcttctgctt gtcggccatg atatagacgt
      361 tgtggctgtt gtagttgtac tccagcttgt gccccaggat gttgccgtcc tccttgaagt
      421 cgatgccctt cagctcgatg cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc
      481 gggtcttgta gttgccgtcg tccttgaaga agatggtgcg ctcctggacg tagccttcgg
      541 gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgc gcgaagcaca
      601 tcaggccgta gccgaaggtg gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg
      661 tgcagatgaa cttcagggtc agcttgccgt aggtggcatc gccctcgccc tcgccggaca
      721 cgctgaactt gtggccgttt acgtcgccgt ccagctcgac caggatgggc accaccccgg
      781 tgaacagctc ctcgccct
//
