LOCUS       sequence-100757-         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Genetically encoded fluorescent voltage indicator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Mishina Y, Mutoh H, Song C, Knopfel T
  TITLE     Exploration of genetically encoded voltage indicators based on a 
            chimeric voltage sensing domain.
  JOURNAL   Front Mol Neurosci. 2014 Sep 29;7:78. doi: 10.3389/fnmol.2014.00078.
            eCollection 2014.
  PUBMED    25324718
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Mol 
            Neurosci."; date: "2014-09-29"; volume: "7:78. doi"; pages: " 
            10.3389/fnmol.2014.00078. eCollection 2014"
COMMENT     Sequence Label: pCAG-Chimeric_Butterfly_CY_1.0
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(74..325)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(116..137)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     465..484
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     724..745
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gcattggcca caccagccac caccttctga taggcagcct gcacctgagg agtgcggccg
       61 ccttaagctt ttacttgtac agctcgtcca tgccgagagt gatcccggcg gcggtcacga
      121 actccagcag gaccatgtga tcgcgcttct cgttggggtc tttgctcagc ttggactggg
      181 tgctcaggta gtggttgtcg ggcagcagca cggggccgtc gccgatgggg gtgttctgct
      241 ggtagtggtc ggcgagctgc acgctgccgt cctcgatgtt gtggcggatc ttgaagttgg
      301 ccttgatgcc gttcttctgc ttgtcggcgg tgatatagac gttgtcgctg atggcgttgt
      361 actccagctt gtgccccagg atgttgccgt cctccttgaa gtcgatgccc ttcagctcga
      421 tgcggttcac cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg tagttgccgt
      481 cgtccttgaa gaagatggtg cgctcctgga cgtagccttc gggcatggcg gacttgaaga
      541 agtcgtgctg cttcatgtgg tcggggtagc gggcgaagca ctgcacgccc caggtcaggg
      601 tggtcacgag ggtgggccag ggcacgggca gcttgccggt ggtgcagatg aacttcaggg
      661 tcagcttgcc gtaggtggca tcgccctcgc cctcgccgga cacgctgaac ttgtggccgt
      721 ttacgtcgcc gtccagctcg accaggatgg gcaccacccc ggtgaacagc tcctcgccct
      781 ttcgacgctt gttctgtgat attgttcttc tcgaggaagc
//
