LOCUS       sequence-100760-        1476 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express Hoxa9 and MeisI to induce leukemic transformation. GFP label
            transduced cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   He J, Nguyen AT, Zhang Y
  TITLE     KDM2b/JHDM1b, an H3K36me2-specific demethylase, is required for 
            initiation and maintenance of acute myeloid leukemia.
  JOURNAL   Blood. 2011 Apr 7;117(14):3869-80. doi: 
            10.1182/blood-2010-10-312736. Epub 2011 Feb 10.
  PUBMED    21310926
REFERENCE   2  (bases 1 to 1476)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1182/blood-2010-10-312736"; journalName: "Blood"; date: 
            "2011-04-7- 7"; volume: "117"; issue: "14"; pages: "3869-80"
COMMENT     Sequence Label: pTY-EF1a-Hoxa9-p2a-MeisI-p2a-GFP
FEATURES             Location/Qualifiers
     source          1..1476
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        288..1472
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          519..1463
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1420..1440
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 caattctcat gtttgacagc ttatcatcga taagctttgg agctaagcca gcaatggtag
       61 agggaagatt ctgcacgtcc cttccaggcg gcctccccgt caccaccccc cccaacccgc
      121 cccgaccgga gctgagagta attcatacaa aaggactcgc ccctgccttg gggaatccca
      181 gggaccgtcg ttaaactccc actaacgtag aacccagaga tcgctgcgtt cccgccccct
      241 cacccgcccg ctctcgtcat cactgaggtg gagaagagca tgcgtgaggc tccggtgccc
      301 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
      361 attgaaccgg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
      421 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
      481 acgttctttt tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc
      541 gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacgcccc
      601 tggctgcagt acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg
      661 aggccttgcg cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc
      721 tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag
      781 tctctagcca tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt
      841 cttgtaaatg cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc
      901 gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca
      961 ccgagaatcg gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg
     1021 ccgccgtgta tcgccccgcc ctgggcggca acaggctggc ccggtcggca ccagttgcgt
     1081 gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc
     1141 gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag
     1201 ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct
     1261 cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc
     1321 cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct
     1381 tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc
     1441 aaagtttttt tcttccattt caggtgtcgt gaaaac
//
