LOCUS       sequence-100766-        1101 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  NpuDnaEN35 intein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Aranko AS, Oeemig JS, Zhou D, Kajander T, Wlodawer A, Iwai H
  TITLE     Structure-based engineering and comparison of novel split inteins 
            for protein ligation.
  JOURNAL   Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h.
  PUBMED    24574026
REFERENCE   2  (bases 1 to 1101)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Biosyst"; date: "2014-05"; volume: "10"; issue: "5"; pages: 
            "1023-34"
COMMENT     Sequence Label: pSARSF53-218
FEATURES             Location/Qualifiers
     source          1..1101
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     317..336
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(356..433)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     479..498
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        479..497
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    498..522
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             578..595
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             602..892
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="SDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLR
                     RLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIETHREQIGG"
     CDS             1029..1073
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 cactgacgcg ttgcggcgag aagattgtgc accgccgctt acaggctcga cgccgcttcg
       61 tctaccatcg acacacacgc tgcacccagt gatcggcgcg agatttaatc gccgcgacaa
      121 tttgcgacgg cgcgtgcagg gccagactgg aggtggcaac gccaatcagc aacgactgtt
      181 tgcccgccag ttgttgtgcc acgcggttgg gaatgtaatt cagctccgcc atcgccgctt
      241 ccactttttc ccgcgttttc gcagaaacgt ggctggcctg gttcaccacg cgggaaacgg
      301 tctgataaga gacaccggca tactctgcga catcgtataa cgttactggt ttcacattca
      361 ccaccctgaa ttgactctct tccgggcgct atcatgccat accgcgaaag gttttgcgcc
      421 attcgatggt gtccgggatc tcgacgctct cccttatgcg actcctgcat taggaaatta
      481 atacgactca ctatagggga attgtgagcg gataacaatt cccctgtaga aataattttg
      541 tttaacttta ataaggagat ataccatggg cagcagccat catcatcatc atcacagcac
      601 tagtgactca gaagtcaatc aagaagctaa gccagaggtc aagccagaag tcaagcctga
      661 gactcacatc aatttaaagg tgtccgatgg atcttcagaa atcttcttta agatcaaaaa
      721 gaccactcct ttaagaaggc tgatggaagc gttcgctaaa agacagggta aggaaatgga
      781 ctccttaaga ttcttgtacg acggtattag aattcaagct gatcagaccc ctgaagattt
      841 ggacatggag gataacgata ttattgagac tcacagagaa cagattggtg gatcctgttt
      901 aagctatgaa acggaaatat tgacagtaga atatggatta ttaccgattg gtaaaattgt
      961 agaaaagcgc atcgaatgta ctgtttatag cgttgataat taagcttgcg gccgcactcg
     1021 agtctggtaa agaaaccgct gctgcgaaat ttgaacgcca gcacatggac tcgtctacta
     1081 gcgcagctat agcatgtaat c
//
