LOCUS       sequence-100768-        1201 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  NpuDnaEN11 intein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Aranko AS, Oeemig JS, Zhou D, Kajander T, Wlodawer A, Iwai H
  TITLE     Structure-based engineering and comparison of novel split inteins 
            for protein ligation.
  JOURNAL   Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h.
  PUBMED    24574026
REFERENCE   2  (bases 1 to 1201)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Biosyst"; date: "2014-05"; volume: "10"; issue: "5"; pages: 
            "1023-34"
COMMENT     Sequence Label: pSARSF53-756
FEATURES             Location/Qualifiers
     source          1..1201
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     489..508
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(528..605)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     651..670
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        651..669
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    670..694
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             750..767
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             774..1064
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="SDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLR
                     RLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIETHREQIGG"
     CDS             1129..1173
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 aaccccaatg cgaccagatg ctcccacgcc ccagtcgcgt accgtcttca tggaagaaaa
       61 taatactgtg atggggttct gtcagagaca tcagaaataa cgccgacata gtgcagcagc
      121 tccacagcaa tggcatcctg gtcatccagc ggatagtatg atcagcccac tgacgcgttg
      181 cgcgagaaga ttgtgcaccg ccgcttacag gcttcgacgc cgcttcgttc taccatcgac
      241 accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac
      301 ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc
      361 agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt
      421 tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa
      481 gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg
      541 aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg
      601 gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaaat taatacgact
      661 cactataggg gaattgtgag cggataacaa ttcccctgta gaaataattt tgtttaactt
      721 taataaggag atataccatg ggcagcagcc atcatcatca tcatcacagc actagtgact
      781 cagaagtcaa tcaagaagct aagccagagg tcaagccaga agtcaagcct gagactcaca
      841 tcaatttaaa ggtgtccgat ggatcttcag aaatcttctt taagatcaaa aagaccactc
      901 ctttaagaag gctgatggaa gcgttcgcta aaagacaggg taaggaaatg gactccttaa
      961 gattcttgta cgacggtatt agaattcaag ctgatcagac ccctgaagat ttggacatgg
     1021 aggataacga tattattgag actcacagag aacagattgg tggatcctgt ttaagctatg
     1081 aaacggaaat attgacagta taagcttgcg gccgcactcg agtctggtaa agaaaccgct
     1141 gctgcgaaat ttgaacgcca gcacatggac tcgtctacta gcgcagctaa tagcatcgta
     1201 t
//
