LOCUS       sequence-100770-        1111 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  NpuDnaB(delta283)dC39 intein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Aranko AS, Oeemig JS, Zhou D, Kajander T, Wlodawer A, Iwai H
  TITLE     Structure-based engineering and comparison of novel split inteins 
            for protein ligation.
  JOURNAL   Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h.
  PUBMED    24574026
REFERENCE   2  (bases 1 to 1111)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Biosyst"; date: "2014-05"; volume: "10"; issue: "5"; pages: 
            "1023-34"
COMMENT     Sequence Label: pSARSF53-259
FEATURES             Location/Qualifiers
     source          1..1111
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     135..154
                     /label=LacI-R
                     /note="LacI, reverse primer"
     primer_bind     296..315
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        296..314
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    315..339
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             395..412
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             419..709
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="SDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLR
                     RLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIETHREQIGG"
     CDS             1038..1082
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 ccacgcatca gcacgactgt tgccgccagt gtgtgcacgc gtgatgtagt cagctcgcat
       61 cgcgctcact tttcccgcgt ttcgcagaaa cgtgctgctg ttcacacgcg ggaaacggtc
      121 tgatagagac accgggcata ctctgcgaca tcgtataacg ttactggttt cacattcacc
      181 acctgaattg actctcttcc cgggcgctat catgccatac cgcgaaaggt tttgcgccat
      241 tcgatggtgt ccgggatctc gacgctctcc ttatgcgact cctgcattag gaaattaata
      301 cgactcacta taggggaatt gtgagcggat aacaattccc ctgtagaaat aattttgttt
      361 aactttaata aggagatata ccatgggcag cagccatcat catcatcatc acagcactag
      421 tgactcagaa gtcaatcaag aagctaagcc agaggtcaag ccagaagtca agcctgagac
      481 tcacatcaat ttaaaggtgt ccgatggatc ttcagaaatc ttctttaaga tcaaaaagac
      541 cactccttta agaaggctga tggaagcgtt cgctaaaaga cagggtaagg aaatggactc
      601 cttaagattc ttgtacgacg gtattagaat tcaagctgat cagacccctg aagatttgga
      661 catggaggat aacgatatta ttgagactca cagagaacag attggtggat ccggttgttt
      721 agcaggcgat agtctggtga cattagtaga tagcggactg caagtgccaa ttaaggaatt
      781 agtaggtaaa tctggttttg cagtttgggc attgaacgaa gctacaatgc agctagaaaa
      841 ggcaattgtt agcaatgctt tttcgactgg tataaagcct ctgtttactt tgacaactcg
      901 attggggcgg aaaatccgtg ccacaggtaa tcacaagttt ttaacaatta atggctggaa
      961 aagacttgat gaattaactc ctaaagaaca tctcagttta ccaagaaatt aagcttgcgg
     1021 ccgcactcga gtctggtaaa gaaaccgctg ctgcgaaatt tgaacgccag cacatggact
     1081 cgtctactag cgcagcctaa tagcatcgca a
//
