LOCUS       sequence-100772-        1192 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  NpuDnaB(delta283)N11 intein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Aranko AS, Oeemig JS, Zhou D, Kajander T, Wlodawer A, Iwai H
  TITLE     Structure-based engineering and comparison of novel split inteins 
            for protein ligation.
  JOURNAL   Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h.
  PUBMED    24574026
REFERENCE   2  (bases 1 to 1192)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Biosyst"; date: "2014-05"; volume: "10"; issue: "5"; pages: 
            "1023-34"
COMMENT     Sequence Label: pSARSF53-HY100
FEATURES             Location/Qualifiers
     source          1..1192
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     472..491
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(511..588)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     634..653
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        634..652
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    653..677
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             733..750
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             757..1047
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="SDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLR
                     RLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIETHREQIGG"
     CDS             1121..1165
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 gctcccaagt cggcggtatc gctctcatgg agaaaatact actgtgatcg agttctagtc
       61 agagacattc caggaatacg ccgatcataa gtgcagccag cttcacagca tgcatcttgt
      121 cattccagcg gatagtaatt gatcagccca ctgacgcgtt gcgcgagaaa gattgtgcac
      181 cgccgcttta cagggcttcg acgccgcttc gttctaccat cgacaccaca cgctggcacc
      241 cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt gcagggccag
      301 actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg
      361 gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga
      421 aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc
      481 tgcgacatcg tataacgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg
      541 gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtccg ggatctcgac
      601 gctctccctt atgcgactcc tgcattagga aattaatacg actcactata ggggaattgt
      661 gagcggataa caattcccct gtagaaataa ttttgtttaa ctttaataag gagatatacc
      721 atgggcagca gccatcatca tcatcatcac agcactagtg actcagaagt caatcaagaa
      781 gctaagccag aggtcaagcc agaagtcaag cctgagactc acatcaattt aaaggtgtcc
      841 gatggatctt cagaaatctt ctttaagatc aaaaagacca ctcctttaag aaggctgatg
      901 gaagcgttcg ctaaaagaca gggtaaggaa atggactcct taagattctt gtacgacggt
      961 attagaattc aagctgatca gacccctgaa gatttggaca tggaggataa cgatattatt
     1021 gagactcaca gagaacagat tggtggatcc ggttgtttag caggcgatag tctggtgaca
     1081 ttagtagata gctaagcttg cggccgcact cgagtctggt aaagaaaccg ctgctgcgaa
     1141 atttgaacgc cagcacatgg actcgtctac tagcgcagct aatagatgcc ac
//
