LOCUS       sequence-100774-        1124 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TvoVMAdC48 intein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Aranko AS, Oeemig JS, Zhou D, Kajander T, Wlodawer A, Iwai H
  TITLE     Structure-based engineering and comparison of novel split inteins 
            for protein ligation.
  JOURNAL   Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h.
  PUBMED    24574026
REFERENCE   2  (bases 1 to 1124)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Biosyst"; date: "2014-05"; volume: "10"; issue: "5"; pages: 
            "1023-34"
COMMENT     Sequence Label: pSARSF53-343
FEATURES             Location/Qualifiers
     source          1..1124
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=LacI-R
                     /note="LacI, reverse primer"
     primer_bind     191..210
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        191..209
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    210..234
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             290..307
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             314..604
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="SDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLR
                     RLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIETHREQIGG"
     CDS             1053..1097
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 tcaccacgcg ggaacggtct gattaagaga acaccggcat actctgcgac atcgtataac
       61 gttactgttt cacattcaca ctgaattgac tctcttccgg gcgctatcat gccataccgc
      121 gaaaggtttg cgcattcgat ggtgtccggg atctcgacgc tctccatatg cgactcctgc
      181 attaggaaat taatacgact cactataggg gaattgtgag cggataacaa ttcccctgta
      241 gaaataattt tgtttaactt taataaggag atataccatg ggcagcagcc atcatcatca
      301 tcatcacagc actagtgact cagaagtcaa tcaagaagct aagccagagg tcaagccaga
      361 agtcaagcct gagactcaca tcaatttaaa ggtgtccgat ggatcttcag aaatcttctt
      421 taagatcaaa aagaccactc ctttaagaag gctgatggaa gcgttcgcta aaagacaggg
      481 taaggaaatg gactccttaa gattcttgta cgacggtatt agaattcaag ctgatcagac
      541 ccctgaagat ttggacatgg aggataacga tattattgag actcacagag aacagattgg
      601 tggatccaag tgcgtatcag gtgaaacacc agtttacctt gcggatggca agacaataaa
      661 aataaaggat ctatacagtt ctgagagaaa aaaagaagat aacattgttg aggctggttc
      721 gggagaagag ataatacatc taaaagatcc cattcaaata tattcttatg tggacgggac
      781 catagtcagg agcagatcaa gacttctata caagggcaag agctcttatc ttgtgaggat
      841 agaaactatt ggcggaagat cggtaagcgt tacaccagtt cacaaactct ttgtccttac
      901 ggaaaagggt atcgaagagg ttatggcctc caacctaaag gtaggcgaca tgattgctgc
      961 tgtagcagaa agcgaatctg aagcaagaga ctgcggaatg agcgaggaat gcgtgatgga
     1021 agcataagct tgcggccgca ctcgagtctg gtaaagaaac cgctgctgcg aaatttgaac
     1081 gccagcacat ggactcgtct actagcgcag ctaatagctg ttta
//
