LOCUS       sequence-100777-        1155 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TvoVMAdC7 intein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Aranko AS, Oeemig JS, Zhou D, Kajander T, Wlodawer A, Iwai H
  TITLE     Structure-based engineering and comparison of novel split inteins 
            for protein ligation.
  JOURNAL   Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h.
  PUBMED    24574026
REFERENCE   2  (bases 1 to 1155)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Biosyst"; date: "2014-05"; volume: "10"; issue: "5"; pages: 
            "1023-34"
COMMENT     Sequence Label: pSARSF53-HY176
FEATURES             Location/Qualifiers
     source          1..1155
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             199..216
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1084..1128
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 accatcaccc acctgaatga ctctcttcgg cctatcatgc atacgcgaaa ggttgcgcat
       61 cgatgtgtcg ggatctgacg ctctgtatgc gactcctgca ttagaaatat acgactcact
      121 atagggatgt gagcggatac atcccctgta gaaataattt gtttaacttt aataaggaga
      181 tataccatgg gcagcagcca tcatcatcat catcacagca ctagtgactc agaagtcaat
      241 caagaagcta agccagaggt caagccagaa gtcaagctga gactcacatc aatttaaagg
      301 tgtccgatgg atcttcagaa atcttcttta agatcaaaaa gaccactcct ttaagaaggc
      361 tgatggaagc gttcgctaaa agacagggta aggaaatgga ctccttaaga ttcttgtacg
      421 acggtattag aattcaagct gatcagaccc ctgaagattt ggacatggag gataacgata
      481 ttattgagac tcacagagaa cagattggtg gatccaagtg cgtatcaggt gaaacaccag
      541 tttaccttgc ggatggcaag acaataaaaa taaaggatct atacagttct gagagaaaaa
      601 aagaagataa cattgttgag gctggttcgg gagaagagat aatacatcta aaagatccca
      661 ttcaaatata ttcttatgtg gacgggacca tagtcaggag cagatcaaga cttctataca
      721 agggcaagag ctcttatctt gtgaggatag aaactattgg cggaagatcg gtaagcgtta
      781 caccagttca caaactcttt gtccttacgg aaaagggtat cgaagaggtt atggcctcca
      841 acctaaaggt aggcgacatg attgctgctg tagcagaaag cgaatctgaa gcaagagact
      901 gcggaatgag cgaggaatgc gtgatggaag cagaagttta tacgtcactt gaagcgacat
      961 tcgatagagt aaagtctata gcgtacgaga agggtgattt tgatgtatac gatctttccg
     1021 taccagaata cggcaggaac tttataggcg gagaataagc ttgcggccgc actcgagtct
     1081 ggtaaagaaa ccgctgctgc gaaatttgaa cgccagcaca tggactcgtc tactagcgca
     1141 gctatagcac gtcac
//
