LOCUS       sequence-100782-         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To construct N-terminal GFP fusions under control of a Zn2+ 
            inducible promoter, integrates via double crossover at bgaA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Eberhardt A, Wu LJ, Errington J, Vollmer W, Veening JW
  TITLE     Cellular localization of choline-utilization proteins in 
            Streptococcus pneumoniae using novel fluorescent reporter systems.
  JOURNAL   Mol Microbiol. 2009 Oct;74(2):395-408. doi: 
            10.1111/j.1365-2958.2009.06872.x. Epub 2009 Sep 8.
  PUBMED    19737355
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1365-2958.2009.06872.x"; journalName: "Mol Microbiol"; 
            date: "2009-10"; volume: "74"; issue: "2"; pages: "395-408"
COMMENT     Sequence Label: pJWV25
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catggatgag ctctacaaat ctagaggatc tggtggagaa gctgcagcta aagctggaac
       61 tagtggagcg gccgcataat aagctgagcg ccggtcgcta ccattaccag ttggtctggt
      121 gtcaaaaata ataataaccg ggcaggccat gtctgcccgt atttcgcgta aggaaatcca
      181 ttatgtacta tttctggtga tgaaatcaac gtaacattta aagctgtcaa agccaaagtc
      241 atgagatggc gtatggagcg taaagctgac aagagcggtg ttgcgatgat tgagatgacc
      301 ttccttgcac caagtgaatt gcctcaagaa agcactcaat caaagattct tgtagatgga
      361 aaagaacttg ctgatttcgc tgaaaatcgt caagactatc aaattaccta taaaggtcaa
      421 cggccaaaag tctcagttga agaaaacaat caagtagctt caactgtggt agatagtgga
      481 gaagatagcc ttccagtact tgttcgcctc gtttcagaaa gtggaaaaca agtcaaggaa
      541 taccgtatcc agttgactaa ggaaaaacca gtttctgctg tacaagaaga tcttccaaaa
      601 ctcgaatttg ttgaaaaaga tttggcctac aagacagttg agaaaaaaga ttcaacactg
      661 tatctaggtg aaactcgtgt agaacaagaa ggaaaagttg gaaaagaacg tatctttaca
      721 gtgattaatc ctgatggaag taaggaagaa aaactccgtg aagtggtaga agttccgaca
      781 gaccgcatcg tcttggttgg aaccaaacca gtagctcaag aagctaaaaa
//
