LOCUS       sequence-100783-         626 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a tandem red ddFP heterodimer in mammalian cells, 
            construct 1 for a red to green colour switch-based fluorescent 
            caspase-3 biosensor, used together with a second plasmid GANLS..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 626)
  AUTHORS   Ding Y, Li J, Enterina JR, Shen Y, Zhang I, Tewson PH, Mo GC, Zhang 
            J, Quinn AM, Hughes TE, Maysinger D, Alford SC, Zhang Y, Campbell RE
  TITLE     Ratiometric biosensors based on dimerization-dependent fluorescent 
            protein exchange.
  JOURNAL   Nat Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261.
  PUBMED    25622108
REFERENCE   2  (bases 1 to 626)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261."
COMMENT     Sequence Label: NESRA-DEVD-BNLS
FEATURES             Location/Qualifiers
     source          1..626
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      90..99
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(298..316)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     566..585
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagctggc tagcgtgccg ccaccatggc ctcgagaatg aacctggtgg
      121 acctccaaaa gaagctggag gagctggagc tggacgagca gcagatggtg agcaagagcg
      181 aggaggtcat caaagagttc atgcgcttca aggtgcgctt ggagggctcc atgaacggcc
      241 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca
      301 agctgaaggt gaccaagggc ggccccctgc ccttcgcctg ggacatcctg tccccccaga
      361 tcatgtacgg ctccaaggcg tacgtgaagc accccgccga cgtccccgat tacatgaagc
      421 tgtccttccc cgagggcttc aagtgggagc gcgtgatgca cttcgaggac ggcggtctgg
      481 tgaccgtgac tcaggacacc tccctgcagg acggcacgct gatctacaag gtgaagatgc
      541 gcggcaccaa cttccccccc gacggccccg taatgcagaa gaagaccttg ggctgggatt
      601 atgccaccga gcgcctgtac cccgaa
//
