LOCUS       sequence-100784-         952 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To construct N-terminal GFP fusions under control of a Zn2+ 
            inducible promoter, integrates via double crossover at bgaA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Eberhardt A, Wu LJ, Errington J, Vollmer W, Veening JW
  TITLE     Cellular localization of choline-utilization proteins in 
            Streptococcus pneumoniae using novel fluorescent reporter systems.
  JOURNAL   Mol Microbiol. 2009 Oct;74(2):395-408. doi: 
            10.1111/j.1365-2958.2009.06872.x. Epub 2009 Sep 8.
  PUBMED    19737355
REFERENCE   2  (bases 1 to 952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1365-2958.2009.06872.x"; journalName: "Mol Microbiol"; 
            date: "2009-10"; volume: "74"; issue: "2"; pages: "395-408"
COMMENT     Sequence Label: pJWV25
FEATURES             Location/Qualifiers
     source          1..952
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ttcttaacgc cccaagttca tcaccaatga catcaactca catgaactac atgatgaacc
       61 cagttatcat ggttttggat aagatttttg aaaaattctt cccaggcctt gataaatatg
      121 actttgatgc tgctaaattg aacaagaaaa tcggtttctg gggatctaaa ttcttcatcg
      181 gtttcatcct tggtatcgtt atcggtatta tgggaactcc acatccaatt gcaggtgttg
      241 cagatgcaga taaatggcgt cttgttatca aaggatggtt gtctcttggt ttgactgccg
      301 gtgtatcttt ggaactcttc tcacttatcg gttcatggtt catcgcagcc gtagaaccac
      361 tatcacaagg tattacaaac gttgctacta aacgtcttca aggacgtaaa ttcaatatcg
      421 gtcttgactg gccattcatc gctggtcgtg ctgaaatctg ggcttgtgcc aacgtacttg
      481 caccaatcat gttgattgaa gcagtgcttc tttcaaaagt tggaaatggt atcttgccac
      541 ttgcaggtat catcgctatg ggtgttactc cagctctctt ggttgtaact cgtggtaaat
      601 tgctccgtat gattatcttc ggaacactct tgttgccact cttccttctt tcaggtacac
      661 ttattgcacc atttgcaaca gaacttgcta aaggtgtagg tgccttccca gaaggtgtga
      721 gccaaactca attgattact cactctactc ttgaaggacc aatcgaaaaa cttcttggtt
      781 ggacaattgg taacactaca actggtgata tcaaagcaat ccttggtgca gtagccttcc
      841 ttgtattcta tatcggtatc tttgcttggt acagaaaaca aatgatcaaa cgtaacgaag
      901 agtacgcagc aaaagcaaaa taatgcgctc cgctagcttt acagacaaag aa
//
