LOCUS       sequence-100790-         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes zinc finger nuclease specific to AAVS1 locus (left).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Sim X, Cardenas-Diaz FL, French DL, Gadue P
  TITLE     A Doxycycline-Inducible System for Genetic Correction of iPSC 
            Disease Models.
  JOURNAL   Methods Mol Biol. 2015 Jan 29.
  PUBMED    25630922
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol. 2015 Jan 29."
COMMENT     Sequence Label: PGK-AAVS1ZFNL
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      45..54
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             57..77
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tcatctccgg gcctttcgac ctgcaagtcg acccgcggac cggtgccacc atggctccaa
       61 agaaaaagcg gaaagtggga atccacggag tccccgccgc tatggcagag agaccttttc
      121 agtgccgaat ttgtatgcgg aacttctctt ataattggca cctgcagagg catatccgca
      181 ctcacaccgg agagaaaccc tttgcatgcg acatttgtgg cagaaagttc gccaggtccg
      241 atcatctgac cacacacact aaaatccata ccggctctca gaagcccttt cagtgccgca
      301 tttgtatgcg aaacttctct cacaattacg cccgggactg ccacatcaga acacatactg
      361 gggagaaacc ttttgcttgc gatatttgtg gacgaaagtt cgcacagaac agtacacgga
      421 tcggccacac taaaattcat ctgagggggt ctcagctggt gaagagtgaa ctggaggaga
      481 agaagagcga gctgcgccac aaactgaagt acgtccctca tgagtatatc gaactgatcg
      541 agattgccag gaattcaacc caggaccgca ttctggaaat gaaagtgatg gagttcttta
      601 tgaaggtcta cggatataga ggcaaacacc tgggcgggtc aaggaagcct gatggcgcta
      661 tctacactgt ggggagccca atcgactatg gagtgattgt cgataccaag gcctacagcg
      721 gaggctataa cctgcccatt ggccaggctg acgagatgga acgctacgtg gaggaaaacc
      781 agacacgaaa caagcatctg aacccaaatg aatggtggaa ggtgtatccc agctccgtca
      841 ctgagttcaa gtttctgttc gtgagcgggc acttt
//
