LOCUS       sequence-100795-         379 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Flag-Tagged nucleoporin Tpr in Mammalian Cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 379)
  AUTHORS   Vomastek T, Iwanicki MP, Burack WR, Tiwari D, Kumar D, Parsons JT, 
            Weber MJ, Nandicoori VK
  TITLE     Extracellular signal-regulated kinase 2 (ERK2) phosphorylation sites
            and docking domain on the nuclear pore complex protein Tpr 
            cooperatively regulate ERK2-Tpr interaction.
  JOURNAL   Mol Cell Biol. 2008 Nov;28(22):6954-66. doi: 10.1128/MCB.00925-08. 
            Epub 2008 Sep 15.
  PUBMED    18794356
REFERENCE   2  (bases 1 to 379)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.00925-08"; journalName: "Mol Cell Biol"; date: 
            "2008-11"; volume: "28"; issue: "22"; pages: "6954-66"
COMMENT     Sequence Label: pCDNA3-Flag-Tpr
FEATURES             Location/Qualifiers
     source          1..379
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      118..127
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             127..150
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttggt accgagctcg gatccactag taacggccgc cagtgtgctg gaattctgcc
      121 accatggact acaaggacga cgatgacaag ccagcggccg ctatggcggc ggtgttgcag
      181 caagtcctgg agcgcacgga gctgaacaag ctgcccaagt ctgtccagaa caaacttgaa
      241 aagttccttg ctgatcagca atccgagatc gatggcctga aggggcggca tgagaaattt
      301 aaggtggaga gcgaacaaca gtattttgaa atagaaaaga ggttgtccca cagtcaggag
      361 agacttgtga atgaaaccc
//
