LOCUS       sequence-100796-         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Reporter construct to study unspliced RNA export. Coding sequence of
            luciferase gene was sandwiched between 5' and 3' splice site 
            sequences originating from the HIV gag/pol gene followed by CTE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Rajanala K, Nandicoori VK
  TITLE     Localization of nucleoporin Tpr to the nuclear pore complex is 
            essential for Tpr mediated regulation of the export of unspliced 
            RNA.
  JOURNAL   PLoS One. 2012;7(1):e29921. doi: 10.1371/journal.pone.0029921. Epub 
            2012 Jan 13.
  PUBMED    22253824
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(1):e29921. doi"; pages: " 
            10.1371/journal.pone.0029921. Epub 2012 Jan 13"
COMMENT     Sequence Label: pcDNA3-CTE-Luc
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(368..386)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct tggtaccgag ctcggatcca ctagtaacgg ccgccagtgt
      121 gctggaattc tgcagatatc catcacactg gcggccgccc ccttcaccgc tagcgaggag
      181 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
      241 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtaagct
      301 tatggaagac gccaaaaaca taaagaaagg cccggcgcca ttctatcctc tggaggatgg
      361 aaccgctgga gagcaactgc ataaggctat gaagagatac gccctggttc ctggaacaat
      421 tgcttttaca gatgcacata tcgaggtgaa catcacgtac gcggaatact tcgaaatgtc
      481 cgttcggttg gcagaagcta tgaaacgata tgggctgaat acaaatcaca gaatcgtcgt
      541 atgcagtgaa aactctcttc aattctttat gccggtgttg ggcgcgttat ttatcggagt
      601 tgcagttgcg cccgcgaacg acatttataa tgaacgtgaa ttgctcaaca gtatgaacat
      661 ttcgcagcct accgtagtgt ttgtttccaa aaaggggttg caaaaaattt tgaacgtgca
      721 aaaaaaatta ccaataatcc agaaaattat tatcatggat tctaaaacgg attaccaggg
      781 atttcagtcg atgtacacgt tcgtcacatc tcatctacct cc
//
