LOCUS       sequence-100805-        1182 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TvoVMAN11 intein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Aranko AS, Oeemig JS, Zhou D, Kajander T, Wlodawer A, Iwai H
  TITLE     Structure-based engineering and comparison of novel split inteins 
            for protein ligation.
  JOURNAL   Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h.
  PUBMED    24574026
REFERENCE   2  (bases 1 to 1182)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Biosyst"; date: "2014-05"; volume: "10"; issue: "5"; pages: 
            "1023-34"
COMMENT     Sequence Label: pSARSF53-110
FEATURES             Location/Qualifiers
     source          1..1182
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     465..484
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(504..581)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     627..646
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        627..645
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    646..670
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             726..743
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             750..1040
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="SDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLR
                     RLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIETHREQIGG"
     CDS             1108..1152
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 cccacggccc agtggcgtac gtcttcatgg agaaaataat actgtgatgg ggtttgtcag
       61 agacatcaga aataacgcga acattagtgc agcagctcac agcaatggca tcctggtcat
      121 ccagcggata gtaatgatca gcccactgac gcgttgcgcg agaagattgt gcacgccgct
      181 ttacaggctt cgacgccgct tcgttctacc atcgacacca ccacgctggc acccagttga
      241 tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc cagactggag
      301 gtggcaacgc caatcagcaa cgactgtttg cccgccagtt gttgtgccac gcggttggga
      361 atgtaattca gctccgccat cgccgcttcc actttttccc gcgttttcgc agaaacgtgg
      421 ctggcctggt tcaccacgcg ggaaacggtc tgataagaga caccggcata ctctgcgaca
      481 tcgtataacg ttactggttt cacattcacc accctgaatt gactctcttc cgggcgctat
      541 catgccatac cgcgaaaggt tttgcgccat tcgatggtgt ccgggatctc gacgctctcc
      601 cttatgcgac tcctgcatta ggaaattaat acgactcact ataggggaat tgtgagcgga
      661 taacaattcc cctgtagaaa taattttgtt taactttaat aaggagatat accatgggca
      721 gcagccatca tcatcatcat cacagcacta gtgactcaga agtcaatcaa gaagctaagc
      781 cagaggtcaa gccagaagtc aagcctgaga ctcacatcaa tttaaaggtg tccgatggat
      841 cttcagaaat cttctttaag atcaaaaaga ccactccttt aagaaggctg atggaagcgt
      901 tcgctaaaag acagggtaag gaaatggact ccttaagatt cttgtacgac ggtattagaa
      961 ttcaagctga tcagacccct gaagatttgg acatggagga taacgatatt attgagactc
     1021 acagagaaca gattggtgga tccaagtgcg tatcaggtga aacaccagtt taccttgcgt
     1081 aagcttgcgg ccgcactcga gtctggtaaa gaaaccgctg ctgcgaaatt tgaacgccag
     1141 cacatggact cgtctactag cgcagctaat agccttgtct gt
//
