LOCUS       sequence-100810-        1125 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PhoRadAdC14 intein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Aranko AS, Oeemig JS, Zhou D, Kajander T, Wlodawer A, Iwai H
  TITLE     Structure-based engineering and comparison of novel split inteins 
            for protein ligation.
  JOURNAL   Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h.
  PUBMED    24574026
REFERENCE   2  (bases 1 to 1125)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Biosyst"; date: "2014-05"; volume: "10"; issue: "5"; pages: 
            "1023-34"
COMMENT     Sequence Label: pSARSF53-166
FEATURES             Location/Qualifiers
     source          1..1125
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     130..149
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        130..148
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    149..173
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             229..246
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             253..543
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="SDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLR
                     RLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIETHREQIGG"
     CDS             1055..1099
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 ggtttcacat tcaccacctg aaattgactc tctcccgggc gctatcatgc cataccgcga
       61 aaagtttgcg ccattcgatg gtgtccggga tctcgacgct ctccttatgc gactcctgca
      121 ttaggaaatt aatacgactc actatagggg aattgtgagc ggataacaat tcccctgtag
      181 aaataatttt gtttaacttt aataaggaga tataccatgg gcagcagcca tcatcatcat
      241 catcacagca ctagtgactc agaagtcaat caagaagcta agccagaggt caagccagaa
      301 gtcaagcctg agactcacat caatttaaag gtgtccgatg gatcttcaga aatcttcttt
      361 aagatcaaaa agaccactcc tttaagaagg ctgatggaag cgttcgctaa aagacagggt
      421 aaggaaatgg actccttaag attcttgtac gacggtatta gaattcaagc tgatcagacc
      481 cctgaagatt tggacatgga ggataacgat attattgaga ctcacagaga acagattggt
      541 ggatccggga agtgctttgc tagggatacc gaagtttatt atgaaaacga tactgtacca
      601 cacatggaat caattgagga gatgtatagt aaatacgcct cgatgaatgg ggaattacca
      661 ttcgataatg gttacgcagt tccattagat aatgtctttg tgtacacgtt ggacatcgct
      721 agtggtgaaa ttaagaaaac gagggcttca tacatctatc gcgagaaggt tgagaagctc
      781 attgagataa aactatccag tggatactcc cttaaggtta ctccctctca cccagttctc
      841 ctctttagag atggcctgca atgggtcccc gctgctgaag ttaaacctgg agatgttgtt
      901 gttggtgtta gggaagaggt actgagaaga agaataatat ccaaaggaga acttgaattc
      961 catgaggttt cctcggtaag gataatagat tacaataact gggtctacga ccttgtaatt
     1021 ccggaataag cttgcggccg cactcgagtc tggtaaagaa accgctgctg cgaaatttga
     1081 acgccagcac atggactcgt ctactagcgc agctatgctc cgcaa
//
