LOCUS       sequence-100812-        1099 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PhoRadAdC6 intein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Aranko AS, Oeemig JS, Zhou D, Kajander T, Wlodawer A, Iwai H
  TITLE     Structure-based engineering and comparison of novel split inteins 
            for protein ligation.
  JOURNAL   Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h.
  PUBMED    24574026
REFERENCE   2  (bases 1 to 1099)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Biosyst"; date: "2014-05"; volume: "10"; issue: "5"; pages: 
            "1023-34"
COMMENT     Sequence Label: pSARSF53-165
FEATURES             Location/Qualifiers
     source          1..1099
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     80..99
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        80..98
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    99..123
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             179..196
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             203..493
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="SDSEVNQEAKPEVKPEVKRETHINLKVSDGSSEIFFKIKKTTPLR
                     RLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIETHREQIGG"
     CDS             1029..1073
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 acggcgaaag ggttttgcgc cattcgatgg tggtccggga tctcgacgct ctccttatgc
       61 gactcctgca ttaggaaatt aatacgactc actatagggg aattgtgagc ggataacaat
      121 tcccctgtag aaataatttt gtttaacttt aataaggaga tataccatgg gcagcagcca
      181 tcatcatcat catcacagca ctagtgactc agaagtcaat caagaagcta agccagaggt
      241 caagccagaa gtcaagcgtg agactcacat caatttaaag gtgtccgatg gatcttcaga
      301 aatcttcttt aagatcaaaa agaccactcc tttaagaagg ctgatggaag cgttcgctaa
      361 aagacagggt aaggaaatgg actccttaag attcttgtac gacggtatta gaattcaagc
      421 tgatcagacc cctgaagatt tggacatgga ggataacgat attattgaga ctcacagaga
      481 acagattggt ggatccggga agtgctttgc tagggatacc gaagtttatt atgaaaacga
      541 tactgtacca cacatggaat caattgagga gatgtatagt aaatacgcct cgatgaatgg
      601 ggaattacca ttcgataatg gttacgcagt tccattagat aatgtctttg tgtacacgtt
      661 ggacatcgct agtggtgaaa ttaagaaaac gagggcttca tacatctatc gcgagaaggt
      721 tgagaagctc attgagataa aactatccag tggatactcc cttaaggtta ctccctctca
      781 cccagttctc ctctttagag atggcctgca atgggtcccc gctgctgaag ttaaacctgg
      841 agatgttgtt gttggtgtta gggaagaggt actgagaaga agaataatat ccaaaggaga
      901 acttgaattc catgaggttt cctcggtaag gataatagat tacaataact gggtctacga
      961 ccttgtaatt ccggaaaccc acaacttcat agctcccaat taagcttgcg gccgcactcg
     1021 agtctggtaa agaaaccgct gctgcgaaat ttgaacgcca gcacatggac tcgtctacta
     1081 gcgcagctaa tacatgtac
//
