LOCUS       sequence-100814-        1175 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PhoRadAN38 intein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Aranko AS, Oeemig JS, Zhou D, Kajander T, Wlodawer A, Iwai H
  TITLE     Structure-based engineering and comparison of novel split inteins 
            for protein ligation.
  JOURNAL   Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h.
  PUBMED    24574026
REFERENCE   2  (bases 1 to 1175)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Biosyst"; date: "2014-05"; volume: "10"; issue: "5"; pages: 
            "1023-34"
COMMENT     Sequence Label: pSARSF53-459
FEATURES             Location/Qualifiers
     source          1..1175
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     378..397
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(417..494)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     540..559
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        540..558
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    559..583
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             639..656
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             663..953
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="SDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLR
                     RLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIETHREQIGG"
     CDS             1105..1149
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 tcaggcaagc tccacacagc aatggcatgc tgtcatccag cgataggtat gatccagtcc
       61 actgacgcgt ggcgggagga aagaattgtg caccgccgcc ttacaggctc gacgccgctc
      121 gtctaccatc gacaccacca cgctgcaccc agtgatcggc gcgagattta atcgccgcga
      181 caatttgcga cggcgcgtgc agggccagac tggaggtggc aacgccaatc agcaacgact
      241 gtttgcccgc cagttgtgtg cacgcggttg ggaatgtaat tcagctccgc catcgccgct
      301 tccacttttt cccgcgtttt cgcagaaacg tggctggcct ggttcaccac gcgggaaacg
      361 gtctgataag agacaccggc atactctgcg acatcgtata acgttactgg tttcacattc
      421 accaccctga attgactctc ttccgggcgc tatcatgcca taccgcgaaa ggttttgcgc
      481 cattcgatgg tgtccgggat ctcgacgctc tcccttatgc gactcctgca ttaggaaatt
      541 aatacgactc actatagggg aattgtgagc ggataacaat tcccctgtag aaataatttt
      601 gtttaacttt aataaggaga tataccatgg gcagcagcca tcatcatcat catcacagca
      661 ctagtgactc agaagtcaat caagaagcta agccagaggt caagccagaa gtcaagcctg
      721 agactcacat caatttaaag gtgtccgatg gatcttcaga aatcttcttt aagatcaaaa
      781 agaccactcc tttaagaagg ctgatggaag cgttcgctaa aagacagggt aaggaaatgg
      841 actccttaag attcttgtac gacggtatta gaattcaagc tgatcagacc cctgaagatt
      901 tggacatgga ggataacgat attattgaga ctcacagaga acagattggt ggatccggga
      961 agtgctttgc tagggatacc gaagtttatt atgaaaacga tactgtacca cacatggaat
     1021 caattgagga gatgtatagt aaatacgcct cgatgaatgg ggaattacca ttcgattaag
     1081 cttgcggccg cactcgagtc tggtaaagaa accgctgctg cgaaatttga acgccagcac
     1141 atggactcgt ctactagcgc agctaaagtt tgctc
//
