LOCUS       sequence-100816-        1162 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PhoRadAN13 intein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Aranko AS, Oeemig JS, Zhou D, Kajander T, Wlodawer A, Iwai H
  TITLE     Structure-based engineering and comparison of novel split inteins 
            for protein ligation.
  JOURNAL   Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h.
  PUBMED    24574026
REFERENCE   2  (bases 1 to 1162)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Biosyst"; date: "2014-05"; volume: "10"; issue: "5"; pages: 
            "1023-34"
COMMENT     Sequence Label: pSARSF53-455
FEATURES             Location/Qualifiers
     source          1..1162
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     440..459
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(479..556)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     602..621
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        602..620
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    621..645
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             701..718
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             725..1015
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="SDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLR
                     RLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIETHREQIGG"
     CDS             1092..1136
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 aagaaaaaat aatacctgtt gatggggttc tgttcagagg acatcaagaa atacgccgac
       61 attagtgcag cagctccaca gcaatggcat cctggtcatc cagcggatag tatgatcagc
      121 ccactgacgc gtgcgcgaga agatgtgcac cgccgcttta caggcttcga cgccgcttcg
      181 tctaccatcg acaccacacg ctggcaccca gttgatcggc gcgagattta atcgccgcga
      241 caatttgcga cggcgcgtgc agggccagac tggaggtggc aacgccaatc agcaacgact
      301 gtttgcccgc cagttgttgt gccacgcggt tgggaatgta attcagctcc gccatcgccg
      361 cttccacttt ttcccgcgtt ttcgcagaaa cgtggctggc ctggttcacc acgcgggaaa
      421 cggtctgata agagacaccg gcatactctg cgacatcgta taacgttact ggtttcacat
      481 tcaccaccct gaattgactc tcttccgggc gctatcatgc cataccgcga aaggttttgc
      541 gccattcgat ggtgtccggg atctcgacgc tctcccttat gcgactcctg cattaggaaa
      601 ttaatacgac tcactatagg ggaattgtga gcggataaca attcccctgt agaaataatt
      661 ttgtttaact ttaataagga gatataccat gggcagcagc catcatcatc atcatcacag
      721 cactagtgac tcagaagtca atcaagaagc taagccagag gtcaagccag aagtcaagcc
      781 tgagactcac atcaatttaa aggtgtccga tggatcttca gaaatcttct ttaagatcaa
      841 aaagaccact cctttaagaa ggctgatgga agcgttcgct aaaagacagg gtaaggaaat
      901 ggactcctta agattcttgt acgacggtat tagaattcaa gctgatcaga cccctgaaga
      961 tttggacatg gaggataacg atattattga gactcacaga gaacagattg gtggatccgg
     1021 gaagtgcttt gctagggata ccgaagttta ttatgaaaac gattaagctt gcggccgcac
     1081 tcgagtctgg taaagaaacc gctgctgcga aatttgaacg ccagcacatg gactcgtcta
     1141 ctagcgcagc taatgcatcg ta
//
