LOCUS       sequence-100820-         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for CRISPR/Cas9 mediated insertion of E2A-KalTA4; to be used in 
            combination with a eGFP specific sgRNA (e.g. Plasmid 61051).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Auer TO, Duroure K, De Cian A, Concordet JP, Del Bene F
  TITLE     Highly efficient CRISPR/Cas9-mediated knock-in in zebrafish by 
            homology-independent DNA repair.
  JOURNAL   Genome Res. 2014 Jan;24(1):142-53. doi: 10.1101/gr.161638.113. Epub 
            2013 Oct 31.
  PUBMED    24179142
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.161638.113"; journalName: "Genome Res"; date: "2014-01";
            volume: "24"; issue: "1"; pages: "142-53"
COMMENT     Sequence Label: eGFPbait-E2A-KalTA4-pA donor vector
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        5..23
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     5..22
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(159..180)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             372..431
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
ORIGIN
        1 agctatttag gtgacactat agaatactca agctatgcat caagcttggt accgagctcg
       61 gatccactag taacggccgc cagtgtgctg gaattcgccc ttatagtggt accatggtga
      121 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
      181 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
      241 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
      301 ccaccctgac ctacggcgtg cagtgcttca gccgctacaa gggcgaattc tgcagatatc
      361 caggaggagg acagtgtact aattatgctc tcttgaaatt ggctggagat gttgagagca
      421 acccaggtcc cggaggagga aaactgctct catccatcga gcaagcctgc gacatttgtc
      481 ggcttaagaa gctgaaatgc tccaaggaaa agccgaaatg tgccaaatgc ctgaagaaca
      541 attgggaatg tcgttactct cccaaaacca agcgaagtcc actcacaagg gctcatctga
      601 ccgaagtgga gagcaggcta gagagactgg aacaactctt tttgctcatc ttccctagag
      661 aggaccttga catgatcctc aagatggatt ctctccagga tattaaagcc cttttgactg
      721 gcttattcgt ccaggacaat gtgaacaaag acgctgtgac agaccgattg gcaagtgtcg
      781 agaccgatat gcctctgaca ctgagacagc acagaatcag cgctacttcc tcaagcgaag
      841 agtcttctaa caagg
//
