LOCUS       sequence-10084-e        4529 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4529)
  TITLE     CHOP plasmids
REFERENCE   2  (bases 1 to 4529)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CHOP 6: mCHOP-WT-9E10-pcDNA1
FEATURES             Location/Qualifiers
     source          1..4529
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     471..490
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     rep_origin      complement(603..1061)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(740..761)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     955..974
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     tRNA            join(1204..1278,1279..1363,1364..1383)
                     /product="E. coli amber-suppressor tyrosine tRNA"
                     /label=supF
     enhancer        1542..1921
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1922..2125
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2076..2096
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2170..2189
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2170..2188
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2226..2255
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     promoter        complement(2801..2819)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2802..2819)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     intron          2903..2968
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             3098..3118
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    3390..3524
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3427..3446)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3481..3500
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4370..4505
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 ggcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
       61 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
      121 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
      181 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
      241 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
      301 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
      361 ctacagcgtg agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
      421 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
      481 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
      541 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcaagcta gcttctagct
      601 agaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
      661 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagcccga
      721 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      781 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc cgcccactac gtgaaccatc
      841 acccaaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
      901 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
      961 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     1021 caccacaccc gccgcgctta atgcgccgct acagggcgcg tactatggtt gctttgacga
     1081 gaccgtataa cgtgctttcc tcgttggaat cagagcggga gctaaacagg aggccgatta
     1141 aagggatttt agacaggaac ggtacgccag ctggattacc gcggtctttc tcaacgtaac
     1201 actttacagc ggcgcgtcat ttgatatgat gcgccccgct tcccgataag ggagcaggcc
     1261 agtaaaagca ttacccgtgg tggggttccc gagcggccaa agggagcaga ctctaaatct
     1321 gccgtcatcg acttcgaagg ttcgaatcct tcccccacca ccatcacttt caaaagtccg
     1381 aaagaatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gtaaaattta
     1441 agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg
     1501 ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag
     1561 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
     1621 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
     1681 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
     1741 ggtggactat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
     1801 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
     1861 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     1921 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
     1981 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
     2041 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
     2101 gtgggaggtc tatataagca gagctctctg gctaactaga gaacccactg cttactggct
     2161 tatcgaaatt aatacgactc actataggga gacccaagct tggtaccgag ctcggatccg
     2221 ccatggagca aaagctgatt tctgaggagg atctggcagc tgagtccctg cctttcacct
     2281 tggagacggt gtccagctgg gagctggaag cctggtatga ggatctgcag gaggtcctgt
     2341 cctcagatga aattgggggc acctatatct catccccagg aaacgaagag gaagaatcaa
     2401 aaaccttcac tactcttgac cctgcgtccc tagcttggct gacagaggag ccagggccaa
     2461 cagaggtcac acgcacatcc caaagccctc gctctccaga ttccagtcag agttctatgg
     2521 cccaggagga agaggaggaa gagcaaggaa gaactaggaa acggaaacag agtggtcagt
     2581 gcccagcccg gcctgggaag caacgcatga aggagaagga gcaggagaac gagcggaaag
     2641 tggcacagct agctgaagag aacgagcggc tcaagcagga aatcgagcgc ctgaccaggg
     2701 aggtggagac cacacggcgg gctctgatcg accgcatggt cagcctgcac caagcatgaa
     2761 cagtgggcat cacctcgagc atgcatctag agggccctat tctatagtgt cacctaaatg
     2821 ctagaggatc tttgtgaagg aaccttactt ctgtggtgtg acataattgg acaaactacc
     2881 tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat gtgttaaact
     2941 actgattcta attgtttgtg tattttagat tccaacctat ggaactgatg aatgggagca
     3001 gtggtggaat gcctttaatg aggaaaacct gttttgctca gaagaaatgc catctagtga
     3061 tgatgaggct actgctgact ctcaacattc tactcctcca aaaaagaaga gaaaggtaga
     3121 agaccccaag gactttcctt cagaattgct aagttttttg agtcatgctg tgtttagtaa
     3181 tagaactctt gcttgctttg ctatttacac cacaaaggaa aaagctgcac tgctatacaa
     3241 gaaaattatg gaaaaatatt tgatgtatag tgccttgact agagatcata atcagccata
     3301 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
     3361 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
     3421 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     3481 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcatcccg ccatggtatc
     3541 aacgccatat ttctatttac agtagggacc tcttcgttgt gtaggtaccg ctgtattcct
     3601 agggaaatag tagaggcacc ttgaactgtc tgcatcagcc atatagcccc cgctgttcga
     3661 cttacaaaca caggcacagt actgacaaac ccatacacct cctctgaaat acccatagtt
     3721 gctagggctg tctccgaact cattacaccc tccaaagtca gagctgtaat ttcgccatca
     3781 agggcagcga gggcttctcc agataaaata gcttctgccg agagtcccgt aagggtagac
     3841 acttcagcta atccctcgat gaggtctact agaatagtca gtgcggctcc cattttgaaa
     3901 attcacttac ttgatcagct tcagaagatg gcggagggcc tccaacacag taattttcct
     3961 cccgactctt aaaatagaaa atgtcaagtc agttaagcag gaagtggact aactgacgca
     4021 gctggccgtg cgacatcctc ttttaattag ttgctaggca acgccctcca gagggcgtgt
     4081 ggttttgcaa gaggaagcaa aagcctctcc acccaggcct agaatgtttc cacccaatca
     4141 ttactatgac aacagctgtt ttttttagta ttaagcagag gccggggacc cctgggcccg
     4201 cttactctgg agaaaaagaa gagaggcatt gtagaggctt ccagaggcaa cttgtcaaaa
     4261 caggactgct tctatttctg tcacactgtc tggccctgtc acaaggtcca gcacctccat
     4321 acccccttta ataagcagtt tgggaacggg tgcgggtctt actccgccca tcccgcccct
     4381 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     4441 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     4501 aggcctaggc ttttgcaaaa agctaattc
//
