LOCUS       sequence-100842-         665 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for generation of a eGFP specific sgRNA from a T7 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Auer TO, Duroure K, De Cian A, Concordet JP, Del Bene F
  TITLE     Highly efficient CRISPR/Cas9-mediated knock-in in zebrafish by 
            homology-independent DNA repair.
  JOURNAL   Genome Res. 2014 Jan;24(1):142-53. doi: 10.1101/gr.161638.113. Epub 
            2013 Oct 31.
  PUBMED    24179142
REFERENCE   2  (bases 1 to 665)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.161638.113"; journalName: "Genome Res"; date: "2014-01";
            volume: "24"; issue: "1"; pages: "142-53"
COMMENT     Sequence Label: DR274-eGFP sgRNA
FEATURES             Location/Qualifiers
     source          1..665
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(32..134)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(135..166)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     primer_bind     214..230
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     214..230
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        359..377
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_RNA        396..471
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 agcaagacgt ttcccgttga atatggctca ttttagcttc cttagctcct gaaaatctcg
       61 ataactcaaa aaatacgccc ggtagtgatc ttatttcatt atggtgaaag ttggaacctc
      121 ttacgtgccg atcaagtcaa aagcctccgg tcggaggctt ttgactttct gctatggagg
      181 tcaggtatga tttaaatggt cagtattgag cctcaggaaa cagctatgac atcaagctga
      241 ctagataatc tagctgatcg tggaccgatc atacgtataa tgccgtaaga tcacgggtcg
      301 cagcacagct cgcggtccag tagtgatcga cactgctcga tccgctcgca ccgctagcta
      361 atacgactca ctataggcga gggcgatgcc acctagtttt agagctagaa atagcaagtt
      421 aaaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg cttttaaaaa
      481 gcttggatcg acgagagcag cgcgactgga tctgtcgccc gtctcaaacg caaccctccg
      541 gcggtcgcat atcattcagg acgagcctca gactccagcg taactggact gcaatcaact
      601 cactggctca ccttccggtc cacgatcagc tagaatcaag ctgactagat aaactggccg
      661 tcgtt
//
